This study investigated the effect of the antioxidant dieckol, a component of Ecklonia cava, on maturation and developmental competence of porcine oocytes exposed to oxidative stress in vitro. Oocytes were matured in in vitro maturation (IVM) medium containing various concentrations of dieckol. The blastocyst formation rate was highest in the 0.5 μM dieckol-treated (0.5 DEK) group. The reactive oxygen species level was decreased, and the level of glutathione and expression of antioxidant genes (NFE2L, SOD1, and SOD2) at metaphase II were increased in the 0.5 DEK group. Abnormal spindle organization and chromosome misalignment were prevented in the 0.5 DEK group. Expression of maternal markers (CCNB1 and MOS) and activity of p44/42 mitogen-activated protein kinase were increased in the 0.5 DEK group. After parthenogenetic activation, the total number of cells per blastocyst was increased and the percentage of apoptotic cells was decreased in the 0.5 DEK group. Expression of development-related genes (CX45, CDX2, POU5F1, and NANOG), antiapoptotic genes (BCL2L1 and BIRC5), and a proapoptotic gene (CASP3) were altered in the 0.5 DEK group. These results indicate that the antioxidant dieckol improves IVM and subsequent development of porcine oocytes and can be used to improve the quality of oocytes under peroxidation experimental conditions.

中文翻译：

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抗氧化剂二烯酚可减少暴露于氧化应激的猪卵母细胞的损伤并促进随后的孤雌生殖胚胎发育

本研究调查了抗氧化剂二甲酚（Ecklonia cava 的一种成分）对暴露于体外氧化应激的猪卵母细胞成熟和发育能力的影响。卵母细胞在含有不同浓度双酚的体外成熟 (IVM) 培养基中成熟。0.5 μM 二烯酚处理 (0.5 DEK) 组的囊胚形成率最高。0.5 DEK组活性氧水平降低，中期II期谷胱甘肽水平和抗氧化基因（NFE2L、SOD1和SOD2）表达增加。0.5 DEK 组防止了异常纺锤体组织和染色体错位。母体标志物（CCNB1和MOS）的表达) 和 p44/42 丝裂原活化蛋白激酶的活性在 0.5 DEK 组中增加。孤雌生殖激活后，0.5 DEK组每个囊胚的细胞总数增加，凋亡细胞百分比降低。的发育相关的基因（表达CX45，CDX2，POU5F1，和NANOG），抗细胞凋亡基因（BCL2L1和BIRC5），和促凋亡基因（CASP3 0.5 DEK组中改变）。这些结果表明，抗氧化剂二甲酚改善了猪卵母细胞的 IVM 和后续发育，可用于改善过氧化实验条件下的卵母细胞质量。