Mineralized tissue regeneration is an important and challenging part of the field of tissue engineering and regeneration. At present, autograft harvest procedures may cause secondary trauma to patients, while bone scaffold materials lack osteogenic activity, resulting in a limited application. Loaded with osteogenic induction growth factor can improve the osteoinductive performance of bone graft, but the explosive release of growth factor may also cause side effects. In this study, we innovatively used platelet-rich fibrin (PRF)-modified bone scaffolds (Bio-Oss^®) to replace autograft, and used cytokine (BMP-2) to enhance osteogenesis. Encouragingly, this mixture, which we named “Autograft Mimic (AGM)”, has multiple functions and advantages. (1) The fiber network provided by PRF binds the entire bone scaffold together, thereby shaping the bone grafts and maintaining the space of the defect area. (2) The sustained release of BMP-2 from bone graft promoted bone regeneration continuously. (3) AGM recruited bone marrow mesenchymal stem cells (BMSCs) and promote their proliferation, migration, and osteogenic differentiation. Thus, AGM developed in this study can improve osteogenesis, and provide new guidance for the development of clinical bone grafts.

矿化的组织再生是组织工程和再生领域中重要且具有挑战性的部分。目前，自体移植物的采集过程可能对患者造成继发性创伤，而骨支架材料缺乏成骨活性，从而导致应用受限。负载成骨诱导生长因子可以改善骨移植物的骨诱导性能，但是生长因子的爆炸性释放也可能引起副作用。在这项研究中，我们创新性地采用富血小板纤维蛋白（PRF）改性的骨支架（BIO-OSS ^®）替代自体移植，并使用细胞因子（BMP-2）增强成骨作用。令人鼓舞的是，这种混合物，我们命名为“ Autograft Mimic（AGM）”，具有多种功能和优点。（1）PRF提供的纤维网络将整个骨支架粘合在一起，从而使骨移植物成形并保持缺损区域的空间。（2）BMP-2从骨移植物中的持续释放持续促进骨再生。（3）AGM募集了骨髓间充质干细胞（BMSC），并促进其增殖，迁移和成骨分化。因此，本研究开发的AGM可以改善成骨作用，并为临床骨移植的发展提供新的指导。