Embryogenic callus induction and regeneration are useful in many aspects of plant biotechnology, especially in the functional characterization of economically important genes. However, in sugarcane, callus induction and regeneration vary across genotypes. Saccharum spontaneum is an important wild germplasm that confers disease resistance and stress tolerance to modern sugarcane cultivars, and its genome has been completely sequenced. The aim of this study was to investigate the effect of genetic variations on embryogenic callus induction and regeneration in S. spontaneum and to screen genotypes having high tissue culture susceptibility. The study was performed using nine genotypes of S. spontaneum and the following five parameters were assessed to determine the response of genotypes to embryogenic callus induction and regeneration: callus induction, embryogenic callus ratio, embryogenic callus induction, embryonic callus regeneration and regeneration capacity. All the genotypes varied significantly (P < 0.01) in all the parameters, except for embryonic callus regeneration, which was high (>80%) for all the genotypes. High broad-sense heritability (86.1–96.8%) indicated that genetic differences are the major source of genotypic variations. Callus induction was found to be strongly positively correlated with embryogenic callus induction (r = 0.890, P < 0.01) and regeneration capacity (r = 0.881, P < 0.01). Among the nine tested genotypes, VN2 was found to be the most responsive to tissue culture and could therefore be used to characterize functional genes in S. spontaneum. We also suggested an approach with potential applications in facilitating the rapid identification of sugarcane genotypes susceptible to tissue culture.

中文翻译：

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自发糖对胚性愈伤组织诱导和再生反应的基因型变异

胚胎愈伤组织诱导和再生在植物生物技术的许多方面都有用，特别是在经济重要基因的功能表征中。然而，在甘蔗中，愈伤组织的诱导和再生因基因型而异。自发糖是一种重要的野生种质，赋予现代甘蔗品种抗病和抗逆能力，其基因组已完全测序。本研究的目的是研究遗传变异对胚胎发生愈伤组织诱导和再生的影响。自发的并筛选具有高组织培养敏感性的基因型。该研究使用九种基因型自发的并评估以下五个参数以确定基因型对胚性愈伤组织诱导和再生的反应：愈伤组织诱导，胚性愈伤组织比率，胚性愈伤组织诱导，胚胎愈伤组织再生和再生能力。所有基因型差异显着（磷< 0.01) 在所有参数中，除了胚胎愈伤组织再生，对于所有基因型来说都很高 (>80%)。高广义遗传力（86.1-96.8%）表明遗传差异是基因型变异的主要来源。发现愈伤组织诱导与胚胎发生愈伤组织诱导呈强正相关。r= 0.890,磷< 0.01) 和再生能力 (r= 0.881,磷< 0.01)。在九个测试的基因型中，发现 VN2 对组织培养最敏感，因此可用于表征自发的. 我们还提出了一种在促进快速鉴定易受组织培养影响的甘蔗基因型方面具有潜在应用的方法。