To establish an efficient regeneration system through somatic embryogenesis of Aralia elata (Miq.) Seem, the effects of explant type and growth regulators on callus induction in A. elata were evaluated. Stems apices were the best explant type for callus induction when cultured on MS medium supplemented with 1.0 mg•L⁻¹ 2,4-D, and the highest induction rate (97.78%) and callus yield (1.72 g of FW•explant⁻¹) were achieved with this medium. Indirect somatic embryogenesis was achieved from calli of the A. elata clone “CH02-1-03”, and the greatest number of somatic embryos (SEs) per callus percent (166.51 SE•g⁻¹ callus) was achieved on MS medium supplemented with 3.0 mg•L⁻¹ IBA in combination with 0.5 mg•L⁻¹ BAP. Somatic embryogenesis and triterpenoid saponins content were significantly influenced by light intensity and the highest of somatic embryogenesis (88.89%) and number of SEs per callus (169.17) were achieved under medium light intensity of 2000 lx. While, total saponins and three saponin monomers content were increased with light intensity. To conveniently isolate SEs and prevent secondary SE regeneration, shoots were cut off and transferred to root regeneration medium; shoots rooted best in MS medium with 4.0 mg•L⁻¹ IBA, with 22.67 average roots number and 9.52 root length higher than other groups. Approximately 81.54% of the cultured plantlets produced roots, and the plantlets exhibited a 81.11% survival rate. .

为了通过Aralia elata（Miq。）体细胞胚发生建立有效的再生系统，似乎，评估了外植体类型和生长调节剂对A. elata愈伤组织诱导的影响。在添加了1.0 mg•L ^-1 2,4-D的MS培养基上培养时，茎尖是诱导愈伤组织的最佳外植体类型，诱导率最高（97.78％）和愈伤组织产量（1.72 g FW•外植体^-1）是使用这种介质实现的。间接体细胞胚发生是通过A. elata克隆的愈伤组织“ CH02-1-03 ”实现的，并且每个愈伤组织百分比的体细胞胚（SEs）数量最多（166.51 SE•g ^-1在补充有3.0 mg•L ^-1 IBA和0.5 mg•L ^-1 BAP的MS培养基上达到愈伤组织）。光照强度对体细胞胚发生和三萜皂苷含量有显着影响，在中等光照强度2000 lx下，体细胞胚发生最高（88.89％）和每个愈伤组织的SE数量（169.17）。同时，总皂苷和三种皂苷单体含量随光强度的增加而增加。为了方便地分离SE和防止SE二次再生，将枝条切下并转移到根再生培养基中。芽最好在4.0 mg•L ^-1的MS培养基中生根IBA，平均根数为22.67，根长比其他组高9.52。约81.54％的培养小植株产生根，小植株的成活率达81.11％。。