This minireview appraises the chemical sensors and biosensors with the bio/sensitive active films obtained by matrix assisted laser evaporation (MAPLE). An overall presentation and examples of the materials utilized thus far introduce the discussion of sensing applications, where the following categories of biosensors are critically appraised: optical sensors (surface plasmon resonance, fluorescence microscopy, long period grating fiber); surface acoustic wave sensors; and electrochemical sensors. The deposition of active layers of biomolecules, potentially useful as sensing interfaces, is discussed in relation to the experimental parameters of MAPLE deposition, with emphasis on the preservation of structure and activity of biomolecules. Additionally, chemoselective layers, temperature responsive and antifouling coatings are also reviewed, as these be can be combined with the active layers to obtain advanced sensing interfaces, showing the versatility of MAPLE. The review concludes with future developments and perspectives.

这次迷你回顾评估了具有通过基质辅助激光蒸发（MAPLE）获得的生物/敏感活性膜的化学传感器和生物传感器。迄今为止所使用的材料的整体介绍和示例介绍了传感应用的讨论，其中对以下类别的生物传感器进行了严格评估：光学传感器（表面等离振子共振，荧光显微镜，长周期光栅纤维）；表面声波传感器；和电化学传感器。与MAPLE沉积的实验参数有关，讨论了可能用作传感界面的生物分子活性层的沉积，重点是保留生物分子的结构和活性。此外，还对化学选择性层，温度响应性涂层和防污涂层进行了回顾，因为这些可以与有源层结合使用以获得先进的传感界面，因此显示了MAPLE的多功能性。审查总结了未来的发展和观点。