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DNA metabarcoding reveals multiple co-occurring species in Stephanodiscus spring diatom blooms in a temperate freshwater river
Marine and Freshwater Research ( IF 1.8 ) Pub Date : 2021-04-06 , DOI: 10.1071/mf20254
Buhari Lawan Muhammad , Yongsik Sin , Jang-Seu Ki

Spring diatom blooms dominate the annual cycle of phytoplankton abundance in temperate freshwater systems. Many researchers have questioned whether severe blooms are attributable to a single species and warrant the identification of similar, concurrently occurring organisms. Here, we investigated spring diatom blooms in a temperate freshwater system (Yeongsan River, South Korea) that is thought to predominantly include the diatom Stephanodiscus hantzschii. Water samples were collected from four different sites during the blooms that occurred in March 2015. We analysed physicochemical and biological parameters and examined the species composition of the spring blooms using DNA metabarcoding via pyrosequencing. Our results revealed that several diatom species co-occurred during the blooms: Cyclotella sp. was the most frequently detected, followed by Cyclostephanos dubius and Discostella sp., whereas Stephanodiscus was only detected at two stations with low occurrence. These results suggest the coexistence of many diatoms during spring blooms in the Yeongsan River; however, Cyclotella sp. was responsible for the spring bloom attributed to S. hantzschii. DNA metabarcoding can be a useful tool for resolving species identities in spring diatom blooms in temperate freshwater systems. In addition, the co-occurrence of similar species should be considered when implementing efforts to monitor and control spring blooms.



中文翻译:

DNA元条形码揭示了温带淡水河中Stephanodiscus春季硅藻盛开的多种同时存在的物种

在温带淡水系统中,春季硅藻的绽放主导着浮游植物丰度的年度循环。许多研究人员质疑严重的水华是否可归因于单个物种,并保证鉴定出类似的同时发生的生物。在这里,我们研究了温带淡水系统(韩国龙山河)中春季硅藻的花朵,据认为主要包括硅藻Stephanodiscus hantzschii。在2015年3月爆发期间,从四个不同的地点收集了水样。我们分析了其理化和生物学参数,并使用了通过焦磷酸测序的DNA元条形码技术对春季开花的物种组成进行了分析。我们的结果表明,在开花过程中同时出现了几种硅藻:Cyclotella sp。被检测到最频繁,其次是杜比Cyclostephanos dubiusDiscostella sp。,而仅在两个发生率较低的站点上检测到Stephanodiscus。这些结果表明,龙山河春季开花期间许多硅藻并存。但是,Cyclotella sp.。造成了汉氏链球菌的春季开花DNA metabarcoding可能是解决温带淡水系统中春季硅藻绽放中物种身份的有用工具。此外,在实施监测和控制春季开花的努力时,应考虑相似物种的同时存在。

更新日期:2021-04-11
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