Old polyester fibre (OPF) was used for the first reported microbial flocculant (MBF) production by Diaphorobacter nitroreducens R9. Pretreatment of OPF was performed by different bases individually. The optimal pretreatment conditions were determined by total organic carbon (TOC) content of hydrolysate supernatants. Although all alkaline OPF-hydrolysate culture media were investigated for MBF production, KOH hydrolysate produced the highest yield. The influence of KOH OPF-hydrolysate culture condition factors were applied throughout the response surface methodology, and the optimal conditions with the maximum MBF yield (4.02 g/L) were 33.5 °C, pH 9.5, and hydrolysate TOC (4.416 g/L). Furthermore, the MBF was applied to treat polyester alkali-peeling wastewater (PAPW) for the first time, and the highest removal efficiency was achieved when 1.0 g/L MBF was added to PAPW. In general, this contribution reveals that KOH OPF-hydrolysate could be used as a potential inexpensive carbon source for production of MBF by sustainable processes that could reduce environmental pollution.

旧的聚酯纤维（OPF）被用于首次报道的硝化丝杆菌R9生产微生物絮凝剂（MBF）。OPF的预处理由不同的碱分别进行。最佳预处理条件由水解产物上清液的总有机碳（TOC）含量确定。尽管对所有碱性OPF水解产物培养基进行了MBF生产研究，但KOH水解产物的收率最高。在整个响应面方法中均采用了KOH OPF水解培养条件因素的影响，具有最大MBF产量（4.02  g / L）的最佳条件为33.5°C，pH 9.5和水解产物TOC（4.416） g / L）。此外，MBF首次用于处理聚酯碱剥离废水（PAPW），当将1.0  g / L MBF添加到PAPW中时，去除效率最高。总的来说，这一贡献表明，KOH OPF水解产物可以用作潜在的廉价碳源，通过可减少环境污染的可持续工艺生产MBF。