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Genetic diversity and distinctness based on morphological and SSR markers in peanut
Agronomy Journal ( IF 2.1 ) Pub Date : 2021-04-10 , DOI: 10.1002/agj2.20671
Yanbin Hong 1 , Manish K. Pandey 2 , Qing Lu 1 , Hao Liu 1 , Sunil S. Gangurde 2 , Shaoxiong Li 1 , Haiyan Liu 1 , Haifen Li 2 , Xuanqiang Liang 1 , Rajeev K. Varshney 2 , Xiaoping Chen 1
Affiliation  

The morphological and molecular diversity of 101 peanut varieties from South China were analyzed to identify distinctness among these varieties. No significant difference was observed for six morphological characteristics whereas a range of 0.25–0.51 of diversity index was observed for 11 morphological characteristics, with an average value of 0.39. Molecular characterization with 40 highly polymorphic simple sequence repeats (SSRs) generated a total of 167 alleles ranging from two to six alleles per marker with average 4.18 alleles per marker. The polymorphism information content (PIC) of these markers varied from 0.79 to 0.26 with an average value of 0.55 per marker. The diversity analysis using morphological and genotyping data grouped all the varieties into seven and six clusters, respectively, and varieties released by the same province tended to be grouped in the same cluster. Mantel testing revealed that the correlations between the similarity coefficient matrixes of the morphological characteristics and SSR markers of different varieties were weak (r = .347), implying that deployment of more SSR markers is needed for achieving distinctness among these peanut varieties. Nevertheless, the combination of morphological characteristics and SSR markers will effectively increase the accuracy of distinctiveness identification.

中文翻译:

基于花生形态和SSR标记的遗传多样性和独特性

对华南101个花生品种的形态和分子多样性进行分析,以确定这些品种之间的独特性。6个形态特征差异不显着,11个形态特征的多样性指数范围为0.25-0.51,平均值为0.39。具有 40 个高度多态性简单序列重复 (SSR) 的分子表征产生了总共 167 个等位基因,每个标记有 2 到 6 个等位基因,每个标记平均有 4.18 个等位基因。这些标记的多态性信息含量 (PIC) 从 0.79 到 0.26 不等,每个标记的平均值为 0.55。使用形态学和基因分型数据进行的多样性分析将所有品种分别分为七个和六个集群,同一省份发布的品种往往属于同一集群。Mantel检验表明,不同品种的形态特征相似系数矩阵与SSR标记之间的相关性较弱(r  = .347),这意味着需要部署更多的 SSR 标记来实现这些花生品种的独特性。然而,形态特征和SSR标记的结合将有效提高显着性识别的准确性。
更新日期:2021-04-10
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