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Nanomedicine perspective in HIV therapy: biosynthesized gold nanoparticles exhibiting enhanced anti-HIV property
Beni-Suef University Journal of Basic and Applied Sciences Pub Date : 2021-04-08 , DOI: 10.1186/s43088-021-00105-y M. R. Kamala Priya , Manickam Ashokkumar , Lucia K. Precilla , Luke Elizabeth Hanna , Priya R. Iyer
Beni-Suef University Journal of Basic and Applied Sciences Pub Date : 2021-04-08 , DOI: 10.1186/s43088-021-00105-y M. R. Kamala Priya , Manickam Ashokkumar , Lucia K. Precilla , Luke Elizabeth Hanna , Priya R. Iyer
Globally, HIV/AIDS is a major public health issue. Currently, available antiretroviral therapy (ART) has a number of impediments. The need for long-term usage of antiretroviral drugs is associated with the problem of drug resistance, high cost, and other side effects. Hence, there is a constant urge for the development of novel drugs to combat the disease. The synthesis of nanoparticles is of great interest because of their reported application in diverse fields. In contrast to chemical synthesis, green synthesis is inexpensive and enviro-safe. Calophyllum inophyllum (CI) is a plant that is known to possess anti-HIV activity. Hence, this plant was exploited for the synthesis of gold nanoparticles (AuNPs) in the present study. Two parts of the plant, namely, the fruit and leaves (CIF and CIL) were used for the synthesis of nanocompounds. The two nanocompounds were tested on HIV-1 isolates at different concentrations and the EC50 values were determined. While CIF-AuNP demonstrated very good anti-HIV activity with an EC50 value of 0.09097 ng/ml, CIL-AuNPs did not show significant anti-HIV activity. The present study is a novel attempt to produce nanocompounds that incorporate the medicinal properties of certain plants that are known to have anti-HIV activity within nanoparticles, such that the compound possesses the attributes of a nanomaterial alongside the phytoactivity. Our results provide evidence to suggest that the CIF-AuNP can be further explored for in vivo activity. In vitro cytotoxicity of the AuNPs was checked in VERO cell lines using the MTT assay. Cytotoxicity was observed to be minimal at all the tested concentrations.
中文翻译:
纳米药物在HIV治疗中的前景:生物合成的金纳米颗粒显示出增强的抗HIV特性
在全球范围内,艾滋病毒/艾滋病是主要的公共卫生问题。当前,可用的抗逆转录病毒疗法(ART)有许多障碍。长期使用抗逆转录病毒药物与耐药性,高成本和其他副作用有关。因此,不断有开发新药来对抗这种疾病的迫切需求。纳米颗粒的合成引起人们极大的兴趣,因为据报道它们在不同领域中的应用。与化学合成相反,绿色合成便宜且对环境安全。Calophyllum inophyllum(CI)是已知具有抗HIV活性的植物。因此,在本研究中,该植物被用于合成金纳米颗粒(AuNPs)。植物的两个部分,即果实和叶子(CIF和CIL)用于合成纳米化合物。在不同浓度的HIV-1分离物中测试了这两种纳米化合物,并确定了EC50值。尽管CIF-AuNP表现出非常好的抗HIV活性,EC50值为0.09097 ng / ml,但CIL-AuNP却没有显示出显着的抗HIV活性。本研究是一种新颖的尝试,以生产结合了某些植物的医学特性的纳米化合物,这些化合物具有已知的抗HIV活性,从而使该化合物除了具有植物活性外,还具有纳米材料的特性。我们的结果提供了证据,表明可以进一步探索CIF-AuNP的体内活性。使用MTT测定法在VERO细胞系中检查了AuNP的体外细胞毒性。在所有测试浓度下观察到的细胞毒性是最小的。
更新日期:2021-04-09
中文翻译:
纳米药物在HIV治疗中的前景:生物合成的金纳米颗粒显示出增强的抗HIV特性
在全球范围内,艾滋病毒/艾滋病是主要的公共卫生问题。当前,可用的抗逆转录病毒疗法(ART)有许多障碍。长期使用抗逆转录病毒药物与耐药性,高成本和其他副作用有关。因此,不断有开发新药来对抗这种疾病的迫切需求。纳米颗粒的合成引起人们极大的兴趣,因为据报道它们在不同领域中的应用。与化学合成相反,绿色合成便宜且对环境安全。Calophyllum inophyllum(CI)是已知具有抗HIV活性的植物。因此,在本研究中,该植物被用于合成金纳米颗粒(AuNPs)。植物的两个部分,即果实和叶子(CIF和CIL)用于合成纳米化合物。在不同浓度的HIV-1分离物中测试了这两种纳米化合物,并确定了EC50值。尽管CIF-AuNP表现出非常好的抗HIV活性,EC50值为0.09097 ng / ml,但CIL-AuNP却没有显示出显着的抗HIV活性。本研究是一种新颖的尝试,以生产结合了某些植物的医学特性的纳米化合物,这些化合物具有已知的抗HIV活性,从而使该化合物除了具有植物活性外,还具有纳米材料的特性。我们的结果提供了证据,表明可以进一步探索CIF-AuNP的体内活性。使用MTT测定法在VERO细胞系中检查了AuNP的体外细胞毒性。在所有测试浓度下观察到的细胞毒性是最小的。