Abstract

This study sought to assess the protective effects of resveratrol and avocado oil in relation to paracetamol-induced hepatotoxicity in rats. The rats were divided into five groups, namely the control, paracetamol (600 mg/kg), resveratrol (RES; 10 mg/kg) + paracetamol, avocado oil (AVO; 200 mg/kg) + paracetamol, and RES + AVO + paracetamol groups. The hepatoprotective activity was evaluated by measuring biochemical parameters such as the total antioxidant status (TAS) and the total oxidant status (TOS) in each rat’s liver homogenates. Any DNA damage was assessed by means of a comet assay. The results showed that the TOS levels were significantly increased in the paracetamol group when compared with the control group. The TOS levels were found to be significantly lower in the paracetamol groups, in comparison with the RES, AVO, and RES + AVO groups. Moreover, the TAS levels significantly increased in the RES and RES + AVO groups when compared with the paracetamol group. The histopathological examination revealed necrotic areas in the rats’ livers. Pretreatment with both RES and RES + AVO was found to reverse the oxidative stress parameters, DNA damage, and necrosis induced by paracetamol. These results suggest that a combination of REV and AVO may protect against paracetamol-induced hepatotoxicity due to their antioxidant properties.

摘要

本研究旨在评估白藜芦醇和鳄梨油对扑热息痛引起的大鼠肝毒性的保护作用。将大鼠分为五组，即对照组、扑热息痛（600 mg/kg）、白藜芦醇（RES；10 mg/kg）+扑热息痛、鳄梨油（AVO；200 mg/kg）+扑热息痛、RES+AVO+对乙酰氨基酚组。通过测量每只大鼠肝脏匀浆中的总抗氧化状态 (TAS) 和总氧化状态 (TOS) 等生化参数来评估保肝活性。通过彗星试验评估任何 DNA 损伤。结果显示，与对照组相比，扑热息痛组的TOS水平显着升高。与 RES、AVO 和 RES + AVO 组相比，扑热息痛组的 TOS 水平显着降低。此外，与扑热息痛组相比，RES 组和 RES + AVO 组的 TAS 水平显着升高。组织病理学检查显示大鼠肝脏中有坏死区域。发现用 RES 和 RES + AVO 进行预处理可以逆转对乙酰氨基酚诱导的氧化应激参数、DNA 损伤和坏死。这些结果表明，由于其抗氧化特性，REV 和 AVO 的组合可以防止对乙酰氨基酚诱导的肝毒性。