The pollen beetle Brassicogethes aeneus (Fabricius, 1775) (Coleoptera: Nitidulidae) is one of the most important pests in oilseed rape (Brassica napus L. var. oleifera Moench) in Czechia. Wide-spread resistance development to pyrethroids in pollen beetle populations was documented in many European countries, including Czechia. The susceptibilities of 10 local pollen beetle populations originating from 10 localities in Czechia to lambda-cyhalothrin, tau-fluvalinate, thiacloprid, indoxacarb and chlorpyrifos were evaluated in 2017–2020 using glass vial bioassays. A high level of resistance to lambda-cyhalothrin was recorded in all the evaluated populations of the pollen beetle. The proportion of the populations resistant to tau-fluvalinate varied between years from 30% to 60%, and in 2018 and 2020, all the tested populations were classified as resistant. Despite the high level of resistance to pyrethroids, a kdr mutation known to cause resistance in the pollen beetle was not detected in any of the 244 tested individuals. In 2017–2020, the first local populations of the pollen beetle with decreased susceptibility to thiacloprid occurred. The LD₉₀ of thiacloprid exceeded the field application dose in 100% and 90% of populations in 2018 and 2020, respectively. All the tested populations were susceptible to indoxacarb and chlorpyrifos. Cross-resistance between lambda-cyhalothrin and thiacloprid and between tau-fluvalinate and thiacloprid was found using data from 2017 to 2020. No cross-resistance between lambda-cyhalothrin and tau-fluvalinate was detected.

花粉甲虫Brassicogethes aeneus（Fabricius，1775）（鞘翅目：Nitidulidae）是捷克菜油菜（Brassica napus L. var。oleifera Moench）中最重要的害虫之一。在包括捷克共和国在内的许多欧洲国家，花粉甲虫中对拟除虫菊酯类药物的广泛耐药性得到了报道。从在捷克的地方10 10本地花粉甲虫种群起源的到敏感性拉姆达-cyhalothrin，tau蛋白-fluvalinate，噻虫啉，茚虫威和毒死蜱是在2017至2020年使用的玻璃小瓶中的生物测定进行评价。高度抗lambda在所有评估的花粉甲虫种群中都记录了-氟氯氰菊酯。对tau- fluvalinate抗药性的人群比例在几年之间从30％到60％不等，并且在2018年和2020年，所有测试人群均被归类为抗药性。尽管对拟除虫菊酯的抗性水平很高，但在244个测试个体中均未检测到已知在花粉甲虫中引起抗性的kdr突变。在2017–2020年，出现了第一个对甲虫啉易感性降低的花粉甲虫当地种群。LD ₉₀在2018年和2020年，噻虫啉的使用率分别超过了100％和90％的人口的田间应用剂量。所有测试人群均对茚虫威和毒死rif敏感。交叉耐药性之间的λ -cyhalothrin和噻虫啉和之间的tau -fluvalinate和噻虫啉，使用数据从2017年到2020年之间无交叉耐药性发现拉姆达-cyhalothrin和tau蛋白检测-fluvalinate。