To study the effects of exogenous metabolic intermediates and antioxidant on Nannochloropsis oculata microalgae strain, the growth performance, biomass productivity, photosynthetic efficiency, and total lipid content were investigated by lab-scale cultivation experiments. Specifically, N. oculata was cultured in f/2 medium supplemented with different concentrations of citric acid or malic acid (0.050, 0.150, 0.750 g L⁻¹) and KI (0.025, 0.050, 0.100, and 0.500 g L⁻¹) to examine the physiological and biochemical effects on the widely distributed marine algae N. oculata. Results showed that the biomass and photosynthetic efficiency showed a downward trend with increasing citric acid and malic acid concentrations, and the low concentrations of metabolic intermediate treatments could enhance the lipid accumulation. The highest lipid content (0.295 or 0.245 g total lipid per gram of dry biomass) was found in cells treated with 0.15 g L^-1 of citric acid and malic acid, which was about 28.45% and 6.99% higher than that growing in the culture lacking metabolic intermediates. Compared with malic acid, citric acid had better effect. Furthermore, potassium iodide (KI), an antioxidant, could also boost the lipid content of N. oculata with slight declining biomass productivity, and the highest lipid content (0.300 g total lipid per gram of dry biomass) was found in cells treated with 0.100 g L^-1 of KI, which was about 28.08% higher than that growing in the culture lacking KI, indicating that KI may be a potential factor to increase the lipid content of algae cells. These findings not only provide insight into the response mechanism of N. oculata to exogenous metabolic intermediates and antioxidant but also help further broaden the key regulatory factors involved in the lipid accumulation within algal cells.

为了研究外源代谢中间体和抗氧化剂对拟南芥微藻菌株的影响，通过实验室规模的培养实验研究了其生长性能，生物量生产力，光合效率和总脂质含量。具体而言，在补充有不同浓度的柠檬酸或苹果酸（0.050、0.150、0.750 g L ^-1）和KI（0.025、0.050、0.100和0.500 g L ^-1）的f / 2培养基中培养N. oculata。研究对广泛分布的海藻N. oculata的生理和生化影响。结果表明，随着柠檬酸和苹果酸浓度的增加，生物量和光合作用效率呈下降趋势，而低浓度的代谢中间处理可促进脂质积累。在用0.15 g L ^-1柠檬酸和苹果酸处理的细胞中发现了最高的脂质含量（每克干生物质总脂质为0.295或0.245 g），这比培养物中生长的脂质高约28.45％和6.99％缺乏代谢中间体。与苹果酸相比，柠檬酸效果更好。此外，碘化钾（KI），一种抗氧化剂，也可以增加眼球猪笼草的脂质含量。生物量生产力略有下降，并且在用0.100 g L ^-1 KI处理的细胞中发现最高的脂质含量（每克干生物质总脂质为0.300 g），比缺乏KI的培养物中的细胞高约28.08％。 ，表明KI可能是增加藻类细胞脂质含量的潜在因素。这些发现不仅为洞肠猪笼草对外源代谢中间体和抗氧化剂的响应机制提供了见识，而且还有助于进一步拓宽涉及藻类细胞内脂质蓄积的关键调控因子。