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Rare earth mine wastewater treatment via modified constructed rapid infiltration system: Nitrogen removal performance and microbial community
Process Safety and Environmental Protection ( IF 7.8 ) Pub Date : 2021-04-10 , DOI: 10.1016/j.psep.2021.04.008
Jiacheng Sun , Chengyuan Su , Ying Xie , Zhi Huang , Jiayi Bao , Menglin Chen , Xiangfeng Lin

The treatment of ion-type rare earth mine (REM) wastewater by a modified constructed rapid infiltration system (CRIS) was explored. Meanwhile, the activities of hydroxylamine oxidase, nitrite reductase and urease in the column were measured, and the microorganisms in the system were analyzed. When the hydraulic load was 0.1 m3 m−2·d-1, the column height had a significant influence on the NH4+-N and TN removal rates. Specifically, the average NH4+-N and TN removal rates were 29.06 % and 14.83 %, respectively, for the CRIS with a 100 cm single-layer inorganic filler layer. While the average NH4+-N and TN removal rates increased to 69.70 % and 38.90 %, respectively, for the CRIS with a 140 cm double-layer inorganic filler layer. After increasing the hydraulic load from 0.1 m3 m−2·d-1 to 0.2 m3 m−2·d-1, the average NH4+-N and TN removal rates increased by 29.61 % and 15.84 %, respectively, in the long CRIS. The activities of hydroxylamine oxidase, nitrite reductase and urease in the column also improved. Nitrite reductase increased to 0.605 nitrite/min mg protein and hydroxylamine oxidase increased to 0.470 U/ mL g in the lower layer of the long CRIS. High-throughput sequencing analysis showed that the dominant bacterial phyla was mainly Proteobacteria and Planctomycetes. Pseudomonas, Bacillus and other aerobic denitrifying bacteria were detected at the genus level, as were the anaerobic denitrifying bacteria Comamonas and Thauera. These results illustrated that aerobic denitrification, anaerobic denitrification and anaerobic ammoxidation simultaneously existed during the CRIS treatment of REM wastewater to achieve TN removal. Furthermore, when using two full-scale CRIS in a series to treat actual REM wastewater, the NH4+-N removal rate reached 99.14 %.



中文翻译:

通过改良的快速渗透系统处理稀土矿废水:脱氮性能和微生物群落

探索了一种改进的构造快速渗透系统(CRIS)处理离子型稀土(REM)废水的方法。同时,测定了色谱柱中羟胺氧化酶,亚硝酸还原酶和脲酶的活性,并对系统中的微生物进行了分析。当水力负荷为0.1 m 3 m -2 ·d -1时,柱高对NH 4 + -N和TN去除率有显着影响。具体而言,对于具有100cm单层无机填料层的CRIS ,平均NH 4 + -N和TN去除率分别为29.06%和14.83%。而平均NH 4 +对于具有140 cm双层无机填料层的CRIS,-N和TN的去除率分别提高到69.70%和38.90%。在将水力负荷从0.1 m 3 m -2 ·d -1增加到0.2 m 3 m -2 ·d -1后,平均NH 4 +在较长的CRIS中,-N和TN的去除率分别增加了29.61%和15.84%。色谱柱中羟胺氧化酶,亚硝酸还原酶和脲酶的活性也得到改善。在长CRIS的下层,亚硝酸盐还原酶增加至0.605亚硝酸盐/ min mg蛋白,羟胺氧化酶增加至0.470 U / mL g。高通量测序分析表明,优势菌门主要是Proteobacteria和Planctomycetes。在属水平上检出了假单胞菌芽孢杆菌和其他好氧反硝化细菌,厌氧反硝化细菌ComamonasThauera也是如此。。这些结果说明,在CRIS处理REM废水以去除TN的过程中,好氧反硝化,厌氧反硝化和厌氧氨氧化同时存在。此外,当使用两个全尺寸CRIS串联处理实际的REM废水时,NH 4 + -N的去除率达到99.14%。

更新日期:2021-04-23
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