Fruit softening, accompanied by cell wall polysaccharides degradation, is a common phenomenon in harvested mango (Mangifera indica L.) fruit. To reduce fruit’s firmness loss and maintain cell wall polysaccharides of mangoes, the effect of electron beam (E-beam) radiation on firmness, cell wall degrading enzyme activities, contents of cell wall polysaccharides, reactive oxygen species (ROS), and the expression of genes involved with fruit softening were studied in harvested mangoes stored at 13 ℃. As compared with untreated fruit, E-beam treated fruit at a dose of 0.5 kGy exhibited higher firmness, higher contents of cell wall polysaccharides, such as sodium carbonate-soluble pectin, hemicellulose and cellulose, as well as a lower content of water-soluble pectin, a lower activity of cell wall degrading enzymes, such as pectin esterase (PE), β-galactosidase (BG), pectate lyase (PEL) and cellulase (CEL), except polygalacturonase (PG), and lower contents of hydrogen peroxide (H₂O₂) and superoxide radical (O₂–) during the storage period. In addition, E-beam radiation also suppressed the expression of MiPE, MiPEL, and MiBG genes. These results suggest that E-beam radiation retards the softening process by reducing the production of H₂O₂ and O₂–, and that they are caused to alternate plant cell wall polysaccharides and retard the transcript level of genes involved in fruit softening in harvested mangoes.

果实软化，伴随着细胞壁多糖的降解，是芒果收获中的常见现象（芒果）L.）水果。为了减少水果的硬度损失并维持芒果的细胞壁多糖，电子束（E-beam）辐射对硬度，细胞壁降解酶活性，细胞壁多糖含量，活性氧（ROS）的表达以及在13℃贮藏的芒果中研究了与果实软化有关的基因。与未处理的水果相比，剂量为0.5 kGy的电子束处理的水果显示出更高的硬度，更高的细胞壁多糖含量，例如碳酸钠可溶的果胶，半纤维素和纤维素，以及更低的水溶性果胶，细胞壁降解酶（如果胶酯酶（PE），β-半乳糖苷酶（BG），果胶酸裂合酶（PEL）和纤维素酶（CEL））的活性较低，但聚半乳糖醛酸酶（PG）除外，而过氧化氢的含量较低（ H在储存期间应含有₂ O ₂）和超氧自由基（O ₂ – ）。此外，电子束辐射还抑制了MiPE，MiPEL和MiBG基因的表达。这些结果表明，电子束辐射通过降低生产h的阻碍软化工序₂ ö ₂和O ₂ - ，和它们引起备用植物细胞壁多糖和延缓涉及水果收获软化的基因的转录水平芒果。