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Absence of progesterone receptor membrane component 1 reduces migration and metastasis of breast cancer
Cell Communication and Signaling ( IF 8.4 ) Pub Date : 2021-04-08 , DOI: 10.1186/s12964-021-00719-w
Sang R Lee 1 , Young Ho Lee 1 , Seong Lae Jo 1 , Jun H Heo 1 , Globinna Kim 2 , Geun-Shik Lee 3 , Beum-Soo An 4 , In-Jeoung Baek 2 , Eui-Ju Hong 1
Affiliation  

Progesterone receptor membrane component 1 (Pgrmc1) is a non-classical progesterone receptor associated with the development of the mammary gland and xenograft-induced breast cancer. Importantly, Pgrmc1 is associated with the expression of estrogen receptor alpha and can be used for predicting the prognosis of breast cancer. Whether the genetic deletion of Pgrmc1 affects the progression of breast cancer is still unclear. We used MMTV-PyMT transgenic mice that spontaneously develop breast tumors. In backcrossed FVB Pgrmc1 knockout (KO) mice, we monitored the development of the primary tumor and lung metastasis. In MCF-7 and MDA-MB-231 tumor cell lines, the migratory activity was evaluated after Pgrmc1 knockdown. There was no significant difference in the development of breast cancer in terms of tumor size at 13 weeks of age between WT and Pgrmc1 KO mice. However, Pgrmc1 KO mice had a significantly longer survival duration compared with WT mice. Furthermore, Pgrmc1 KO mice exhibited a significantly lower degree of lung metastasis. Compared with those of WT mice, the tumors of Pgrmc1 KO mice had a low expression of focal adhesion kinase and epithelial–mesenchymal transition markers. PGRMC1 knockdown resulted in a significantly reduced migration rate in breast cancer cell lines. Pgrmc1 KO mice with breast cancer had a prolonged survival, which was accompanied by a low degree of lung metastasis. PGRMC1 showed a significant role in the migration of breast cancer cells, and may serve as a potential therapeutic target in breast cancer.

中文翻译:

孕酮受体膜成分 1 的缺失减少了乳腺癌的迁移和转移

孕酮受体膜成分 1 (Pgrmc1) 是一种非经典孕酮受体,与乳腺和异种移植物诱导的乳腺癌的发展有关。重要的是,Pgrmc1与雌激素受体α的表达有关,可用于预测乳腺癌的预后。Pgrmc1的基因缺失是否会影响乳腺癌的进展仍不清楚。我们使用了自发形成乳腺肿瘤的 MMTV-PyMT 转基因小鼠。在回交的 FVB Pgrmc1 敲除 (KO) 小鼠中,我们监测了原发性肿瘤和肺转移的发展。在 MCF-7 和 MDA-MB-231 肿瘤细胞系中,在 Pgrmc1 敲低后评估迁移活性。WT 和 Pgrmc1 KO 小鼠在 13 周龄时的乳腺癌发展没有显着差异。然而,与 WT 小鼠相比,Pgrmc1 KO 小鼠的存活时间明显更长。此外,Pgrmc1 KO 小鼠的肺转移程度显着降低。与 WT 小鼠相比,Pgrmc1 KO 小鼠的肿瘤中粘着斑激酶和上皮间质转化标志物的表达较低。PGRMC1 敲低导致乳腺癌细胞系的迁移率显着降低。患有乳腺癌的 Pgrmc1 KO 小鼠的生存期延长,并伴有低程度的肺转移。PGRMC1在乳腺癌细胞迁移中显示出重要作用,可能作为乳腺癌的潜在治疗靶点。与 WT 小鼠相比,Pgrmc1 KO 小鼠的存活时间明显更长。此外,Pgrmc1 KO 小鼠的肺转移程度显着降低。与 WT 小鼠相比,Pgrmc1 KO 小鼠的肿瘤中粘着斑激酶和上皮间质转化标志物的表达较低。PGRMC1 敲低导致乳腺癌细胞系的迁移率显着降低。患有乳腺癌的 Pgrmc1 KO 小鼠的生存期延长,并伴有低程度的肺转移。PGRMC1在乳腺癌细胞迁移中显示出重要作用,可能作为乳腺癌的潜在治疗靶点。与 WT 小鼠相比,Pgrmc1 KO 小鼠的存活时间明显更长。此外,Pgrmc1 KO 小鼠的肺转移程度显着降低。与 WT 小鼠相比,Pgrmc1 KO 小鼠的肿瘤中粘着斑激酶和上皮间质转化标志物的表达较低。PGRMC1 敲低导致乳腺癌细胞系的迁移率显着降低。患有乳腺癌的 Pgrmc1 KO 小鼠的生存期延长,并伴有低程度的肺转移。PGRMC1在乳腺癌细胞迁移中显示出重要作用,可能作为乳腺癌的潜在治疗靶点。与 WT 小鼠相比,Pgrmc1 KO 小鼠的肿瘤中粘着斑激酶和上皮间质转化标志物的表达较低。PGRMC1 敲低导致乳腺癌细胞系的迁移率显着降低。患有乳腺癌的 Pgrmc1 KO 小鼠的生存期延长,并伴有低程度的肺转移。PGRMC1在乳腺癌细胞迁移中显示出重要作用,可能作为乳腺癌的潜在治疗靶点。与 WT 小鼠相比,Pgrmc1 KO 小鼠的肿瘤中粘着斑激酶和上皮间质转化标志物的表达较低。PGRMC1 敲低导致乳腺癌细胞系的迁移率显着降低。患有乳腺癌的 Pgrmc1 KO 小鼠的生存期延长,并伴有低程度的肺转移。PGRMC1在乳腺癌细胞迁移中显示出重要作用,可能作为乳腺癌的潜在治疗靶点。
更新日期:2021-04-08
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