Objective

Recent studies revealed the neuroprotective effects of hyperbaric oxygen (HBO) on spinal cord injury (SCI). Meanwhile, the use of methylprednisolone (MP) is one of the current protocols with limited effects in SCI patients. Accordingly, the aim of the present study was to investigate the effect of combined HBO and MP treatment on SCI.

Design

The present study was conducted on five groups of rats each as follows: Sham group (underwent laminectomy alone at T9 level vertebra); SCI group (underwent moderate contusive SCI); MP group (underwent SCI and received MP); HBO group (underwent SCI and received HBO); HBO + MP group (underwent SCI and simultaneously received MP and HBO). Blood serum and Spinal cord tissue samples were taken 48 h after SCI for analysis of serum ferric reducing antioxidant power (FRAP) and tissue malodialdehyde (MDA) levels as well as immunohistochemistry of caspase-3 and tumor necrosis factor-alpha (TNF-α). Neurological function was evaluated by the Basso–Beattie–Bresnehan (BBB) locomotion scores until the end of experiments. Additionally, histopathology was assessed at the end of the study.

Setting

Mazandaran University of Medical Sciences, Sari, Iran.

Results

Combination therapy with HBO and MP in the HBO + MP group significantly decreased MDA as well as increased FRAP levels compared to other treatment groups. Meanwhile, attenuated TNF-α and Caspase-3 expression could be significantly detected in the HBO + MP group. At the end of treatment, the neurological outcome was significantly improved and the extent of injured spinal tissue was also significantly reduced in the HBO + MP compared to other treatment groups.

Conclusion

The results suggest that combined therapy with MP and HBO has synergistic effects on SCI treatment.

中文翻译：

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高压氧与甲基强的松龙对大鼠挫伤性脊髓损伤后神经保护的协同作用

客观的

Recent studies revealed the neuroprotective effects of hyperbaric oxygen (HBO) on spinal cord injury (SCI). Meanwhile, the use of methylprednisolone (MP) is one of the current protocols with limited effects in SCI patients. Accordingly, the aim of the present study was to investigate the effect of combined HBO and MP treatment on SCI.

Design

本研究对五组大鼠进行，每组如下：SCI 组（经历中度挫伤性 SCI）；MP组（做过SCI，收到MP）；HBO组（做过SCI，收过HBO）；HBO+MP组（SCI同时接受MP和HBO）。SCI 后 48 小时采集血清和脊髓组织样本，用于分析血清铁还原抗氧化能力 (FRAP) 和组织丙二醛 (MDA) 水平以及 caspase-3 和肿瘤坏死因子-α (TNF-α) 的免疫组织化学. 通过 Basso–Beattie–Bresnehan (BBB) 运动评分评估神经功能，直至实验结束。此外，在研究结束时评估了组织病理学。

环境

马赞达兰医科大学，伊朗萨里。

结果

与其他治疗组相比，HBO + MP 组中 HBO 和 MP 的联合治疗显着降低了 MDA 并增加了 FRAP 水平。同时，在 HBO + MP 组中可以显着检测到减弱的 TNF-α 和 Caspase-3 表达。在治疗结束时，与其他治疗组相比，HBO + MP 组的神经学结果显着改善，脊柱组织损伤的程度也显着减少。

结论

结果表明，MP和HBO联合治疗对SCI治疗具有协同作用。