Alpinia eremochlamys K. Schum, Etlingera flexuosa A.D. Poulsen, and Etlingera acanthoides A.D. Poulsen are endemic Zingiberaceae plants from Central Sulawesi, Indonesia. This study is the first report on screening the potential antiviral activity of ethanol extracts of the leaves, pseudostems, and rhizomes parts on HIV-infected MT-4 cells and identifying chemical constituents by GC-MS. The plants were extracted by the maceration method using 96% ethanol as a solvent. The antiviral activity was measured using Viral-ToxGlo colorimetric method and using the extracts at concentrations ranging from 7.8 to 1000 μg/mL. GC-MS was used to identify the secondary metabolites of potential extracts. The results showed that ethanol extract of E. acanthoides rhizome was the most potent antiviral activity (IC₅₀ of 1.74 ± 2.46 μg/mL) and less toxic on lymphocyte (MT-4) cells (CC₅₀ of 204.90 ± 106.35 μg/mL), affording the highest value of selectivity index (SI) of 117.76. A. eremochlamys rhizomes also showed promising antiviral activity with IC₅₀ of 64.18 ± 2.58 μg/mL and no toxicity on MT-4 cells affording a high SI value 19.05. Preliminary GC-MS identification showed the presence of terpenoids and fatty acids as major compounds. Zerumbone, ar-turmerone, caryophyllene, and caryophyllene oxide were also detected. Chemical constituents identified by GC-MS might be responsible for the antiviral activity of extracts, suggesting further isolation and antiviral testing of the purified compounds.

Alpinia eremochlamys K. Schum ，Etlingera flexuosa AD Poulsen和Etlingera acanthoides AD Poulsen是来自印度尼西亚苏拉威西中部的特有姜科植物。这项研究是首次报道了叶，假茎和根茎部分的乙醇提取物对HIV感染的MT-4细胞的潜在抗病毒活性的筛选，并通过GC-MS鉴定了化学成分。通过浸渍法使用96％乙醇作为溶剂提取植物。抗病毒活性使用Viral-ToxGlo比色法和浓度为7.8至1000μg/ mL的提取物进行测量。GC-MS用于鉴定潜在提取物的次生代谢产物。结果表明，刺五加乙醇提取物根茎是最有效的抗病毒活性（IC ₅₀为1.74± 2.46μg/ mL），对淋巴细胞（MT-4）细胞的毒性较小（CC ₅₀为204.90±106.35μg/ mL），具有最高的选择性指数（SI） ）的117.76。A. eremochlamys的根茎还显示出有希望的IC ₅₀抗病毒活性64.18±2.58μg/ mL的浓度，对MT-4细胞无毒性，提供19.05的高SI值。初步的GC-MS鉴定表明存在萜类化合物和脂肪酸作为主要化合物。还检测到了Zerumbone，ar-turmerone，石竹烯和石竹烯氧化物。通过GC-MS鉴定的化学成分可能与提取物的抗病毒活性有关，这表明对纯化的化合物进行了进一步的分离和抗病毒测试。