Oxygen deficit and sulfide have been restrictive factors in mariculture zones. However, the adaptive mechanism in aquatic lives is still unclear. The commercial ark shells Anadara broughtonii were selected to test the tolerance and adaptive responses to prolonged and intermittent hypoxia with or without exogenous sulfide (mild, moderate, high) by evaluating their behavior, mortality, oxidative level, antioxidant responses, and the MAPK-mediated apoptosis in gills. The results indicated that the clams were tolerant to hypoxia and sulfide exposure but vulnerable during reoxygenation from the challenges. Even so, sulfide had remarkable effect on attenuating the accumulation of reactive oxygen species (ROS) and lipid peroxides caused by reoxygenation from prolonged hypoxia. The increase of glutathione level was probably as an early and primary protective response to prevent the expected reperfusion injury from reoxygenation. The challenges suppressed the oxidative level with a dose-dependent effect of sulfide, with an exception when exposed to mild sulfide. Synchronously, biphasic effects of exogenous sulfide on apoptotic cascade, which was induced by mild sulfide while it was inhibited by higher sulfide, were also detected in gills. The induced or inhibited apoptosis by hypoxia and sulfide kept to a typical ROS-MAPK-CASPASE cascade, desiderating further investigation.

缺氧和硫化物已成为海水养殖区的限制因素。但是，水生生物的适应机制仍不清楚。商用方舟贝壳Anadara Brownii通过评估其行为，死亡率，氧化水平，抗氧化反应以及M中MAPK介导的细胞凋亡，选择用于检测有或没有外源硫化物（轻度，中度，高度）的长期和间歇性缺氧的耐受性和适应性反应。结果表明，蛤were耐受低氧和硫化物的暴露，但在挑战的复氧过程中很脆弱。即便如此，硫化物在减缓长期缺氧引起的再氧化所引起的活性氧（ROS）和脂质过氧化物的积累方面具有显着的效果。谷胱甘肽水平的升高可能是早期和主要的保护性反应，以防止预期的再灌注损伤。这些挑战通过硫化物的剂量依赖性效应抑制了氧化水平，暴露于轻度硫化物时例外。同时，在g中还检测到外源性硫化物对凋亡级联反应的两相作用，轻度硫化物诱导了凋亡的级联反应，而高等硫化物则抑制了凋亡的级联反应。低氧和硫化物诱导或抑制的细胞凋亡维持在典型的ROS-MAPK-CASPASE级联反应中，有待进一步研究。