当前位置: X-MOL 学术Appl. Phys. Express › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Visualization of water concentration distribution in human skin by ultra-multiplex coherent anti-Stokes Raman scattering (CARS) microscopy
Applied Physics Express ( IF 2.3 ) Pub Date : 2021-04-07 , DOI: 10.35848/1882-0786/abee9f
Daiki Kaneta 1 , Masahiro Kimura 1 , Philippe Leproux 2 , Vincent Couderc 2 , Makiko Goto 3 , Mariko Egawa 3 , Hideaki Kano 1, 4
Affiliation  

Ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging was used to visualize the distribution of water concentration in human skin ex vivo. The CARS signal of the OH stretching vibrational mode of water was found to coexist with the signal of intercellular lipids such as ceramides, which were visualized by a sharp vibrational band at 2882cm−1. Depth-resolved CARS spectroscopic imaging of a skin sample revealed that ceramides were localized in the stratum corneum. These findings demonstrate the powerful potential of CARS spectroscopic imaging for probing pathological changes caused by anomalous water concentration distribution in human skin.



中文翻译:

通过超多重相干反斯托克斯拉曼散射 (CARS) 显微镜观察人体皮肤中的水浓度分布

超多重相干反斯托克斯拉曼散射 (CARS) 光谱成像用于可视化离体人体皮肤中水浓度的分布。发现水的 OH 伸缩振动模式的 CARS 信号与细胞间脂质(如神经酰胺)的信号共存,在 2882cm -1处通过尖锐的振动带可视化。皮肤样本的深度分辨 CARS 光谱成像显示神经酰胺位于角质层中。这些发现证明了 CARS 光谱成像在探测人体皮肤中异常水浓度分布引起的病理变化方面的强大潜力。

更新日期:2021-04-07
down
wechat
bug