当前位置: X-MOL 学术J. Pharm. Anal. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Analysis of anticancer compound, indole-3-carbinol, in broccoli using a new ultrasound-assisted dispersive-filter extraction method based on poly(deep eutectic solvent)-graphene oxide nanocomposite
Journal of Pharmaceutical Analysis ( IF 8.8 ) Pub Date : 2021-04-07 , DOI: 10.1016/j.jpha.2021.03.013
Yanan Yuan 1, 2 , Huanhuan Chen 1 , Yehong Han 2 , Fengxia Qiao 3 , Hongyuan Yan 1, 2
Affiliation  

Indole-3-carbinol (I3C), an important anticancer compound found in broccoli, has attracted considerable attention. The rapid extraction and accurate analysis of I3C in the pharmaceutical industry in broccoli is challenging as I3C is unstable at low pH and high temperature. In this study, a rapid, accurate, and low-cost ultrasound-assisted dispersive-filter extraction (UADFE) technique based on poly(deep eutectic solvent)-graphene oxide (PDES-GO) adsorbent was developed for the isolation and analysis of I3C in broccoli for the first time. PDES-GO with multiple adsorption interactions and a fast mass transfer rate was synthesized to accelerate adsorption and desorption. UADFE was developed by combining dispersive solid-phase extraction (DSPE) and filter solid-phase extraction (FSPE) to realize rapid extraction and separation. Based on the above two strategies, the proposed PDES-GO-UADFE method coupled with high-performance liquid chromatography (HPLC) allowed the rapid (15–16 min), accurate (84.3%–96.4%), and low-cost (adsorbent: 3.00 mg) analysis of I3C in broccoli and was superior to solid-phase extraction, DSPE, and FSPE methods. The proposed method showed remarkable linearity (r=0.9998; range: 0.0840–48.0 μg/g), low limit of quantification (0.0840 μg/g), and high precision (relative standard deviation ≤5.6%). Therefore, the PDES-GO-UADFE-HPLC method shows significant potential in the field of pharmaceutical analysis for the separation and analysis of anti-cancer compounds in complex plant samples.



中文翻译:

使用基于聚(低共熔溶剂)-氧化石墨烯纳米复合材料的新型超声辅助分散过滤萃取方法分析西兰花中的抗癌化合物吲哚-3-甲醇

吲哚-3-甲醇(I3C)是西兰花中发现的一种重要的抗癌化合物,引起了广泛的关注。在制药行业中,西兰花中 I3C 的快速提取和准确分析具有挑战性,因为 I3C 在低 pH 和高温下不稳定。在本研究中,开发了一种基于聚(低共熔溶剂)-氧化石墨烯(PDES-GO)吸附剂的快速、准确且低成本的超声辅助分散过滤萃取(UADFE)技术,用于分离和分析 I3C第一次在西兰花中。合成了具有多重吸附相互作用和快速传质速率的PDES-GO,以加速吸附和解吸。UADFE是将分散固相萃取(DSPE)和过滤固相萃取(FSPE)相结合而开发出来的,可实现快速萃取分离。基于上述两种策略,所提出的 PDES-GO-UADFE 方法与高效液相色谱(HPLC)相结合,可以快速(15-16 分钟)、准确(84.3%-96.4%)和低成本(吸附剂) :3.00 mg)对西兰花中的 I3C 进行分析,优于固相萃取、DSPE 和 FSPE 方法。该方法表现出显着的线性(r =0.9998;范围:0.0840-48.0 μg/g)、定量限低(0.0840 μg/g)和高精度(相对标准偏差≤5.6%)。因此,PDES-GO-UADFE-HPLC方法在复杂植物样品中抗癌化合物的药物分析领域的分离和分析中显示出巨大的潜力。

更新日期:2021-04-07
down
wechat
bug