ABSTRACT

Type III effectors play a vital role in the establishment of symbiosis. In this study, positive and negative effects of NopT on nodulation were detected in 30 soybean germplasms. The quantitative trait loci (QTL) related to nodule number were identified and their responses to NopT in recombinant inbred lines and chromosome segment substitution lines were evaluated. Ten QTL were identified on chromosomes 02, 05, 06, 10, and 13. One overlapping region on chromosome 02 was responsive to the wild-type rhizobium and the NopT mutant. The expression patterns of the genes in this overlapping region were determined and single nucleotide polymorphisms and haplotypes were analyzed to identify the candidate genes. Four candidate genes were identified as significantly responsive to NopT. Glyma.02G135100 and Glyma.02G100800 expression levels were directly affected by NopT. These results form the basis of future efforts to identify the plant host genes affected by type III effectors.

摘要

III型效应子在建立共生中起着至关重要的作用。在这项研究中，在30种大豆种质中检测到NopT对结瘤的正反作用。鉴定了与根瘤数量有关的数量性状基因座（QTL），并评估了它们对重组自交系和染色体区段替代系中NopT的反应。在10号，02号，05号，06号，10号和13号染色体上鉴定出10个QTL。02号染色体上的一个重叠区域对野生型根瘤菌和NopT突变体有反应。确定该重叠区域中基因的表达模式，并分析单核苷酸多态性和单倍型以鉴定候选基因。鉴定出四个候选基因对NopT有显着反应。Glyma.02G135100和Glyma.02G100800的表达水平直接受NopT影响。这些结果构成了未来努力确定受III型效应子影响的植物宿主基因的基础。