Strawberries (Fragaria × ananassa Duch. cv. Akihime) were treated with different concentrations of exogenous strigolactone (SL) (0, 0.5, 1, 2, and 4 μmol L⁻¹). Changes in the antioxidant system, nitric oxide (NO) synthesis, hydrogen sulfide (H₂S) synthesis, and phenylpropanoid metabolism of postharvest strawberries were studied. Among the treatments, 1 μmol L⁻¹ SL significantly inhibited peroxidase and polyphenol oxidase activities, and enhanced the ability to scavenge ·DPPH, ·OH, and ·O₂^·-. Treatment with 1 μmol L⁻¹ SL effectively reduced weightlessness rate and respiratory intensity, maintained the water content, inhibited the increase in POD activity and the decrease in the activities of CAT and SOD, maintained the reduced ascorbic acid, and reduced glutathione contents. Exogenous SL effectively increased the activities of 4-coumaric acid coenzyme A ligase and phenylalanine ammonia-lyase, thus promoting the accumulation of flavonoids, lignin, and total phenols. These findings suggested that SL alleviated the oxidative damage of strawberries by improving the defense capability of the antioxidant system. SL improved nitric oxide synthase (NOS)-like activity and the contents of l-arginine and endogenous NO, but had no significant effects on nitrate reductase activity and nitrite content, suggesting that SL regulates the synthesis of NO mainly through the NOS-like pathway. Exogenous SL promoted the cleavage of l-cysteine by activating l-cysteine desulfhydrase, d-cysteine desulfhydrase, O-acetylserine thiolyase, and serine acetyltransferase, thus increasing endogenous H₂S content in strawberries. SL maintained fruit quality by improving the antioxidative system and the metabolism of phenylpropanoid, NO, and H₂S in strawberries during storage.

用不同浓度的外源松香内酯（SL）（0、0.5、1、2和4μmolL ^-1）处理草莓（Fragaria×ananassa Duch。cv。Akihime ）。研究了草莓采后抗氧化系统，一氧化氮（NO）合成，硫化氢（H ₂ S）合成和苯丙烷代谢的变化。在这些处理中，1μmolL ^-1 SL显着抑制过氧化物酶和多酚氧化酶活性，并增强清除·DPPH，·OH和·O ₂ ^{··的能力}。用1μmolL ^-1处理SL有效降低失重率和呼吸强度，维持水分含量，抑制POD活性的增加以及CAT和SOD活性的降低，维持抗坏血酸的减少，并减少谷胱甘肽的含量。外源SL有效地增加了4-香豆酸辅酶A连接酶和苯丙氨酸氨裂解酶的活性，从而促进了类黄酮，木质素和总酚的积累。这些发现表明SL通过提高抗氧化剂系统的防御能力减轻了草莓的氧化损伤。SL提高了一氧化氮合酶（NOS）样活性和l的含量-精氨酸和内源性NO，但对硝酸还原酶活性和亚硝酸盐含量没有显着影响，这表明SL主要通过类似NOS的途径调节NO的合成。外源SL通过激活1-半胱氨酸脱硫酶，d-半胱氨酸脱硫酶，O-乙酰丝氨酸硫解酶和丝氨酸乙酰转移酶来促进1-半胱氨酸的裂解，从而增加了草莓中的内源性H ₂ S含量。SL通过改善草莓在贮藏期间的抗氧化系统以及苯丙氨酸，NO和H ₂ S的代谢来保持水果品质。