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SPL36 Encodes a Receptor-like Protein Kinase that Regulates Programmed Cell Death and Defense Responses in Rice
Rice ( IF 5.5 ) Pub Date : 2021-04-07 , DOI: 10.1186/s12284-021-00475-y
R. A. O. Yuchun , J. I. A. O. Ran , W. A. N. G. Sheng , W. U. Xianmei , Y. E. Hanfei , P. A. N. Chenyang , L. I. Sanfeng , Xin Dedong , Z. H. O. U. Weiyong , D. A. I. Gaoxing , H. U. Juan , R. E. N. Deyong , W. A. N. G. Yuexing

Lesion mimic mutants spontaneously produce disease spots in the absence of biotic or abiotic stresses. Analyzing lesion mimic mutants’ sheds light on the mechanisms underlying programmed cell death and defense-related responses in plants. Here, we isolated and characterized the rice (Oryza sativa) spotted leaf 36 (spl36) mutant, which was identified from an ethyl methanesulfonate-mutagenized japonica cultivar Yundao population. spl36 displayed spontaneous cell death and enhanced resistance to rice bacterial pathogens. Gene expression analysis suggested that spl36 functions in the disease response by upregulating the expression of defense-related genes. Physiological and biochemical experiments indicated that more cell death occurred in spl36 than the wild type and that plant growth and development were affected in this mutant. We isolated SPL36 by map-based cloning. A single base substitution was detected in spl36, which results in a cysteine-to-arginine substitution in SPL36. SPL36 is predicted to encode a receptor-like protein kinase containing leucine-rich domains that may be involved in stress responses in rice. spl36 was more sensitive to salt stress than the wild type, suggesting that SPL36 also negatively regulates the salt-stress response. These findings suggest that SPL36 regulates the disease resistance response in rice by affecting the expression of defense- and stress-related genes.



中文翻译:

SPL36编码受体样蛋白激酶,调节水稻中编程的细胞死亡和防御反应。

在没有生物或非生物胁迫的情况下,病变模拟突变体会自发产生病斑。分析病变模拟突变体揭示了植物中程序性细胞死亡和防御相关反应的潜在机制。在这里,我们分离并鉴定了水稻(Oryza sativa斑点叶36spl36)突变体,该突变体是从甲磺酸乙酯诱变的粳稻云道种群中鉴定的。spl36显示出自发性细胞死亡并增强了对水稻细菌病原体的抗性。基因表达分析提示spl36通过上调防御相关基因的表达在疾病反应中发挥功能。生理和生化实验表明,在spl36中发生的细胞死亡多于野生型,并且该突变体影响了植物的生长发育。我们通过基于图的克隆分离了SPL36。在检测到一个碱基置换spl36,这导致半胱氨酸至精氨酸置换在SPL36。预计SPL36编码一种受体样蛋白激酶,其中包含富含亮氨酸的结构域,该结构域可能参与水稻的胁迫反应。spl36对盐胁迫的敏感性高于野生型,表明SPL36还负面地调节了盐胁迫反应。这些发现表明,SPL36通过影响防御和胁迫相关基因的表达来调节水稻的抗病性反应。

更新日期:2021-04-08
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