Present knowledge on the quantitative aerobic physiology of the yeast Saccharomyces cerevisiae during growth on sucrose as sole carbon and energy source is limited to either adapted cells or to the model laboratory strain CEN.PK113-7D. To broaden our understanding of this matter and open novel opportunities for sucrose-based biotechnological processes, we characterized three strains, with distinct backgrounds, during aerobic batch bioreactor cultivations. Our results reveal that sucrose metabolism in S. cerevisiae is a strain-specific trait. Each strain displayed distinct extracellular hexose concentrations and invertase activity profiles. Especially, the inferior maximum specific growth rate (0.21 h-1) of the CEN.PK113-7D strain, with respect to that of strains UFMG-CM-Y259 (0.37 h-1) and JP1 (0.32 h-1), could be associated to its low invertase activity (0.04–0.09 U/mgDM). Moreover, comparative experiments with glucose or fructose alone, or in combination, suggest mixed mechanisms of sucrose utilization by the industrial strain JP1, and points out the remarkable ability of the wild isolate UFMG-CM-259 to grow faster on sucrose than on glucose in a well-controlled cultivation system. This work hints to a series of metabolic traits that can be exploited to increase sucrose catabolic rates and bioprocess efficiency.

中文翻译：

---

酿酒酵母对蔗糖的有氧生长生理是菌株依赖性的

目前关于酵母酿酒酵母在以蔗糖作为唯一碳源和能源的生长过程中的定量需氧生理学的知识仅限于适应细胞或模型实验室菌株 CEN.PK113-7D。为了拓宽我们对这一问题的理解并为基于蔗糖的生物技术过程开辟新的机会，我们在需氧批量生物反应器培养过程中对三种具有不同背景的菌株进行了表征。我们的研究结果表明，酿酒酵母中的蔗糖代谢是一种菌株特异性性状。每个菌株显示出不同的细胞外己糖浓度和转化酶活性谱。特别是 CEN.PK113-7D 菌株的最大比生长速率 (0.21 h-1) 低于 UFMG-CM-Y259 (0.37 h-1) 和 JP1 (0.32 h-1) 菌株，可能与其低转化酶活性（0.04-0.09 U/mgDM）有关。此外，单独或组合使用葡萄糖或果糖的比较实验表明，工业菌株 JP1 利用蔗糖的混合机制，并指出野生分离株 UFMG-CM-259 在蔗糖上比在葡萄糖上生长更快的显着能力一个控制良好的种植系统。这项工作暗示了一系列可用于提高蔗糖分解代谢率和生物过程效率的代谢特征。并指出野生分离株 UFMG-CM-259 在控制良好的培养系统中在蔗糖上比在葡萄糖上生长更快的显着能力。这项工作暗示了一系列可用于提高蔗糖分解代谢率和生物过程效率的代谢特征。并指出野生分离株 UFMG-CM-259 在控制良好的培养系统中在蔗糖上比在葡萄糖上生长更快的显着能力。这项工作暗示了一系列可用于提高蔗糖分解代谢率和生物过程效率的代谢特征。