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An inexpensive, versatile, compact, programmable temperature controller and thermocycler for simultaneous analysis and visualization within a microscope
Microfluidics and Nanofluidics ( IF 2.8 ) Pub Date : 2021-04-07 , DOI: 10.1007/s10404-021-02440-z
Pablo Martínez Cruz , Mikayla A. Wood , Reha Abbasi , Thomas B. LeFevre , Stephanie E. McCalla

Microfluidic Lab on a Chip (LOC) devices are key enabling technologies for research and industry due to their compact size, which increases the number of integrated operations while decreasing reagent use. Common operations within these devices such as chemical and biological reactions, cell growth, or kinetic measurements often require temperature control. Commercial temperature controllers are constrained by cost, complexity, size, and especially versatility for use in a broad range of applications. Small companies and research groups need temperature control systems that are more accessible, which have a wide applicability. This work describes the fabrication and validation of an inexpensive, modular, compact, and user-friendly temperature control system that functions within a microscope. This system provides precise temperature acquisition and control during imaging of any arbitrary sample which complies with the size of a microscope slide. The system includes two parts. The first part is a compact and washable Device Holder that is fabricated from high-temperature resistant material and can fit securely inside a microscope stage. The second part is a robust Control Device that incorporates all the necessary components to program the temperature settings on the device and to output temperature data. The system can achieve heating and cooling times between 50 °C and 100 °C of 32 s and 101 s, respectively. A Bluetooth enabled smartphone application has been developed for real-time data visualization. The utility of the temperature control system was shown by monitoring rhodamine B fluorescence in a microfluidic device over a range of temperatures, and by performing a polymerase chain reaction (PCR) within a microscope. This temperature control system could potentially impact a broad scope of applications that require simultaneous imaging and temperature control.



中文翻译:

廉价,多功能,紧凑,可编程的温度控制器和热循环仪,可在显微镜内同时进行分析和可视化

微流控芯片实验室(LOC)设备具有紧凑的尺寸,是研究和工业应用的关键技术,既增加了集成操作的数量,又减少了试剂的使用。这些设备中的常见操作(例如化学和生物反应,细胞生长或动力学测量)通常需要温度控制。商业温度控制器受成本,复杂性,尺寸,尤其是在广泛应用中的多功能性的限制。小型公司和研究小组需要更易于访问且具有广泛适用性的温度控制系统。这项工作描述了在显微镜内工作的廉价,模块化,紧凑且用户友好的温度控制系统的制造和验证。该系统可在对任何符合显微镜载玻片尺寸的任意样品进行成像时提供精确的温度采集和控制。该系统包括两个部分。第一部分是紧凑且可清洗的设备支架,该支架由耐高温材料制成,可以牢固地安装在显微镜载物台内部。第二部分是强大的控制设备,其中包含所有必需的组件,以对设备上的温度设置进行编程并输出温度数据。该系统可以在50℃和100℃之间分别实现32 s和101 s的加热和冷却时间。已开发了支持蓝牙的智能手机应用程序,用于实时数据可视化。通过在一定温度范围内监控微流体设备中的若丹明B荧光,并在显微镜内进行聚合酶链反应(PCR),可以显示温度控制系统的实用性。这种温度控制系统可能会影响要求同时成像和温度控制的广泛应用范围。

更新日期:2021-04-08
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