Traditional uses of plants are one of the important starting points in search of newer and more effective bioactive drug. Rosa brunonii (plant’s family; Rosaceae) is an ethnomedicinal shrub found in lesser Himalaya region. In traditional medicines, it is used as “blood purifier, to stimulate wound healing, to treat infectious diseases”. So, by considering the medicinal potential of Rosa brunonii, the methanolic fruit’s extract was tested against two gram positive and three gram negative bacteria by agar well diffusion method. The maximum antibacterial potential was noticed against Klebsiella pneumoniae (94 ± 0.3 mm inhibition zone, MIC = 62.5 ± 1.1 µg/mL), while minimum antibacterial activity was shown by the plant extract against Bacillus subtilis (MIC = 250 ± 7.2 µg/mL). The cytotoxic properties of crude methanolic extract were determined by brine shrimp lethality test. A dose-dependent cytotoxic effect was observed for the plant extract at p < 0.05, F (3,8) = 1.58 with LD₅₀ 220.95 ± 1.03 µg/mL. Antitumor potential was evaluated by potato disk antitumor bioassay. Fruit’s extract of R. brunonii showed maximum tumor inhibition at 1000 µg/mL by 72.73% with IC₅₀ = 655.65 ± 0.03 µg/mL. Preliminary phytochemical analysis revealed that extract is devoid of alkaloid but rich in phenolics. Total phenolic and flavonoid contents were determined as 66.73 ± 3.89 µg/mg GAE and 46.51 ± 0.96 µg/mg QE, respectively, in R. brunonii. The present study underlined the ethnomedicinal importance of R. brunonii and promised useful candidate for the search of newer and effective bioactive constituents.

植物的传统用途是寻找更新，更有效的生物活性药物的重要起点之一。Rosa brunonii（植物科；蔷薇科）是在喜马拉雅山较小地区发现的一种民族药用灌木。在传统医学中，它被用作“血液净化剂，刺激伤口愈合，治疗传染病”。因此，考虑到罗莎（Rosa brunonii）的药用潜力，通过琼脂井扩散法对含甲醇水果的提取物针对2克阳性和3克阴性细菌进行了测试。观察到对肺炎克雷伯菌具有最大的抗菌潜力（94±0.3 mm抑制区，MIC = 62.5±1.1 µg / mL），而植物提取物对肺炎克雷伯菌则显示出最小的抗菌活性。枯草芽孢杆菌（MIC = 250±7.2 µg / mL）。粗甲醇提取物的细胞毒性通过盐水虾杀伤力试验确定。在p  <0.05，F（3,8）= 1.58，LD ₅₀ 220.95±1.03 µg / mL时，观察到植物提取物的剂量依赖性细胞毒性作用。通过马铃薯盘抗肿瘤生物测定法评估抗肿瘤潜力。布鲁诺氏菌的果实提取物在1000 µg / mL时显示出最大的肿瘤抑制率，为72.73％，IC ₅₀  = 655.65±0.03 µg / mL。初步的植物化学分析表明，提取物不含生物碱，但富含酚类物质。布鲁诺氏菌中的总酚和类黄酮含量分别为66.73±3.89 µg / mg GAE和46.51±0.96 µg / mg QE 。。本研究强调了布鲁氏红杆菌的民族学意义，并有望为寻找更新有效的生物活性成分提供有用的候选物。