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Characterization of multiple metal resistant Bacillus licheniformis and its potential use in arsenic contaminated industrial wastewater
Applied Water Science ( IF 5.5 ) Pub Date : 2021-04-07 , DOI: 10.1007/s13201-021-01407-3
Shahid Sher , Sikander Sultan , Abdul Rehman

In the present study, the arsenic bioremediation ability of Bacillus licheniformis (dubbed as A6) was determined. The strain was isolated from metal polluted wastewater and was identified on the basis of 16S rRNA sequence homology with accession number of KX 785,171. The bacterium showed resistance against multiple toxic heavy metals, and MIC against arsenic was 3000 µg/ml. Resistance of the bacterium against other toxic metal ions was 3000 µg/ml (Cr), 50 µg/ml (Hg), 1000 µg/ml (Mn), 4000 µg/ml (Se), 500 µg/ml (Pb), 100 µg/ml (Co), 70 µg/ml (Cd) and 100 µg/ml (Zn). The optimum growth temperature was 37 °C while pH was 7. The strain also showed resistance against commonly used antibiotics except ceftriaxone 30 µg and amoxicillin with clavulanic acid (2:1) 3 µg. B. licheniformis could oxidize arsenite into arsenate 86 and 98% after 48 and 96 h from the medium at optimum growth conditions. Due to its high oxidation potential, B. licheniformis can be used in the biological treatment of wastewater containing arsenic.



中文翻译:

多金属抗性地衣芽孢杆菌的表征及其在砷污染工业废水中的潜在应用

在本研究中,测定了地衣芽孢杆菌(被称为A6)的砷生物修复能力。从金属污染的废水中分离出该菌株,并根据16S rRNA序列同源性进行了鉴定,保藏号为KX 785,171。该细菌显示出对多种有毒重金属的抵抗力,对砷的MIC为3000 µg / ml。细菌对其他有毒金属离子的抵抗力为3000 µg / ml(Cr),50 µg / ml(Hg),1000 µg / ml(Mn),4000 µg / ml(Se),500 µg / ml(Pb), 100 µg / ml(Co),70 µg / ml(Cd)和100 µg / ml(Zn)。最佳生长温度为37°C,pH为7。该菌株还显示出对常用抗生素的抗药性,除了头孢曲松30 µg和阿莫西林与克拉维酸(2:1)3 µg。地衣芽孢杆菌在最佳生长条件下,可从培养基中培养48和96小时后,将砷氧化成86%和98%的砷酸盐。由于地衣芽孢杆菌具有很高的氧化潜能,因此可用于含砷废水的生物处理。

更新日期:2021-04-08
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