Abstract

We previously demonstrated that the histone deacetylase inhibitor, trichostatin A (TSA), improves the development of cat-cow interspecies somatic cell nuclear transfer (iSCNT) embryos. In this study, we investigated if 50 nM TSA treatment for 24 h would improve the rate of embryo development to the blastocyst stage in cat-pig iSCNT embryos, compared with cat-cow iSCNT embryos under same treatment. Despite the SCNT technique (Piezo technique) differing from our previous study (conventional technique), we confirmed that TSA supplementation at 50 nM improved the development of cat-cow iSCNT blastocysts. Porcine oocytes showed the capability to support cat iSCNT embryo development up to the blastocyst stage without TSA treatment although bovine oocytes did not. The blastocyst developmental rate of TSA treated cat-pig iSCNT embryos was significantly lower than that of the non-treated groups (0.68 and 7.95%, respectively, p < 0.05). We used in vitro-fertilized (IVF) porcine embryos to compare the effects of acetylation levels on H3K9 between cat-pig iSCNT embryos and naturally fertilized embryos. We found no differences in acetylation levels of H3K9 between the TSA treated and non-treated iSCNT groups, and the levels were lower than that of IVF embryos. In conclusion, the effects of TSA treatment on iSCNT embryo production were found to be species-specific and dependent on individual characteristics.

中文翻译：

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Trichostatin A处理Cat-Cow和Cat-Pig种间体细胞核移植胚之间的胚泡发育比较

摘要

我们以前证明组蛋白脱乙酰基酶抑制剂曲古抑菌素A（TSA）可以改善猫-牛种间体细胞核移植（iSCNT）胚胎的发育。在这项研究中，我们调查了与同等处理的猫牛iSCNT胚胎相比，用50 nM TSA处理24 h是否能提高猫猪iSCNT胚胎的胚胎发育到胚泡阶段的速率。尽管SCNT技术（压电技术）与我们先前的研究（常规技术）不同，但我们确认以50 nM的剂量补充TSA可以改善猫母牛iSCNT囊胚的发育。猪卵母细胞显示出支持猫iSCNT胚胎发育直至胚泡阶段的能力，而无需TSA处理，尽管牛卵母细胞却没有。p＜0.05）。我们使用体外受精（IVF）猪胚胎比较了猫猪iSCNT胚胎和自然受精胚胎之间乙酰化水平对H3K9的影响。我们发现，经TSA处理和未处理的iSCNT组之间，H3K9的乙酰化水平没有差异，并且该水平低于IVF胚胎。总之，发现TSA处理对iSCNT胚胎产生的影响是物种特异性的，并取决于个体特征。