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Screening of Macrofungi Isolates for Aflatoxin B 1 and Ochratoxin A Degradation
Biology Bulletin ( IF 0.5 ) Pub Date : 2021-04-06 , DOI: 10.1134/s1062359021020126
Tuncay Söylemez , Mustafa Yamaç

Abstract

In the present study, ninety-four macro fungi isolates were screened for aflatoxin B1 and ochratoxin A degradation potential by their culture filtrates. Decreasing toxin amounts were determined by a semi-quantitative HPTLC method. The degradation capacity of the selected ten macrofungi isolates for each toxin was confirmed additionally by High Performance Liquid Chromatography (HPLC). The most successful isolates were determined as Lentinus strigosus OBCC 5004 and Agaricus campestris OBCC 5048 for aflatoxin B1 and ochratoxin A degradation, respectively. Both of these species were reported as a first time for their mycotoxin degradation. The determined aflatoxin B1 degradation rates of Lentinus strigosus with HPTLC and HPLC were 66.70 and 67.00%, respectively. On the other hand, the ochratoxin A degradation rates of Agaricus campestris were recorded as 63.20 and 56%, for both methods, respectively. Toxicity evaluation of non-degraded and degraded aflatoxin B1 and ochratoxin A was performed by using the brine shrimp (Artemia salina) lethality assay, where 20.00 and 60.00% detoxification rates were observed for degraded AFB1 and OTA.



中文翻译:

黄曲霉毒素B 1和O曲毒素A降解的大型真菌分离物的筛选

摘要

在本研究中,通过培养滤液筛选了94株大型真菌分离株的黄曲霉毒素B 1和曲霉毒素A降解潜力。通过半定量HPTLC方法确定毒素的减少量。通过高效液相色谱法(HPLC)进一步确定了所选十种大型真菌分离物对每种毒素的降解能力。确定最成功的分离株分别是用于黄曲霉毒素B 1和曲霉毒素A降解的Strentius strigosus OBCC 5004和Camparis campestris OBCC 5048 。据报道,这两种物种都是首次被霉菌毒素降解。确定的黄曲霉毒素B 1的降解速率用HPTLC和HPLC测得的Lentinus strigosus分别为66.70%和67.00%。另一方面,两种方法的姬松茸中曲霉毒素A降解率分别记录为63.20和56%。通过盐水虾(卤虫)的致死率测定对未降解和降解的黄曲霉毒素B 1和曲霉毒素A进行了毒性评估,其中降解的AFB 1和OTA的解毒率分别为20.00和60.00%。

更新日期:2021-04-08
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