当前位置: X-MOL 学术Biomed. Microdevices › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
3D printed alginate bead generator for high-throughput cell culture
Biomedical Microdevices ( IF 2.8 ) Pub Date : 2021-04-05 , DOI: 10.1007/s10544-021-00561-4
Donghee Lee 1 , Sydney E Greer 1 , Mitchell A Kuss 2 , Yang An 3 , Andrew T Dudley 1
Affiliation  

Alginate hydrogel beads are a common platform for generating 3D cell cultures in biomedical research. Simple methods for bead generation using a manual pipettor or syringe are low-throughput and produce beads showing high variability in size and shape. To address these challenges, we designed a 3D printed bead generator that uses an airflow to cleave beads from a stream of hydrogel solution. The performance of the proposed alginate bead generator was evaluated by changing the volume flow rates of alginate (QAlg) and air (QA), the diameter of device nozzle (d) and the concentration of alginate gel solution (C). We identified that the diameter of beads (D = 0.9 -2.8 mm) can be precisely controlled by changing QA and d. Also the bead generation frequency (f) can be tuned by changing QAlg. Finally, we demonstrated that viability and biological function (pericellular matrix deposition) of chondrocytes were not adversely affected by high f using this bead generator. Because 3D printing is becoming a more accessible technique, our unique design will allow greater access to average biomedical research laboratories, STEM education and industries in cost- and time-effective manner.



中文翻译:

用于高通量细胞培养的 3D 打印藻酸盐珠发生器

海藻酸盐水凝胶珠是在生物医学研究中生成 3D 细胞培养物的常用平台。使用手动移液器或注射器生成珠粒的简单方法是低通量,并且产生的珠粒在大小和形状上表现出高度可变性。为了应对这些挑战,我们设计了一个 3D 打印的珠子发生器,它使用气流从水凝胶溶液流中切割珠子。通过改变藻酸盐 ( Q Alg ) 和空气 ( Q A )的体积流量、装置喷嘴的直径 ( d ) 和藻酸盐凝胶溶液 ( C ) 的浓度来评估所提出的藻酸盐珠发生器的性能。我们确定珠子的直径(D = 0.9 -2.8 mm) 可以通过改变Q Ad来精确控制。此外,可以通过改变Q Alg来调整珠子生成频率 ( f ) 。最后,我们证明了软骨细胞的活力和生物学功能(细胞外基质沉积)不会受到使用这种珠发生器的高f的不利影响。由于 3D 打印正在成为一种更容易获得的技术,我们独特的设计将使普通生物医学研究实验室、STEM 教育和行业以具有成本效益和时间效益的方式获得更多的机会。

更新日期:2021-04-06
down
wechat
bug