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Effect of cortisol on the immune-like response of rainbow trout (Oncorhynchus mykiss) myotubes challenged with Piscirickettsia salmonis
Veterinary Immunology and Immunopathology ( IF 1.8 ) Pub Date : 2021-04-06 , DOI: 10.1016/j.vetimm.2021.110240
Victoria Carrizo 1 , Cristián A Valenzuela 2 , Rodrigo Zuloaga 1 , Camila Aros 3 , Claudia Altamirano 4 , Juan A Valdés 5 , Alfredo Molina 5
Affiliation  

Salmonids are a species of high commercial value in Chilean aquaculture, where muscle is the final product of the industry. Fish can be affected by stress during intensive cultures, increasing susceptibility to infections. Recently, we reported that muscle is an important focus of immune reactions. However, studies have shown the immunosuppressive effect of stress only in lymphoid organs, and few studies have been conducted on muscle and immunity. Hence, we determine the effects of cortisol on the immune-like response of fish myotubes challenged with Piscirickettsia salmonis by three trials. First, rainbow trout primary culture of muscle was cultured and treated with cortisol (100 ng/mL) for 3 and 4 h. Second, myotubes were challenged with P. salmonis (MOI 50) for 4, 6 and 8 h. And third, muscle cell cultures were pretreated with cortisol and then challenged with P. salmonis. The mRNA levels of glucocorticoid pathway and innate immunity were evaluated by qPCR. Cortisol increased the klf15 levels and downregulated the innate immune-related tlr5m gene and antimicrobial peptides. P. salmonis challenge upregulated several immune-related genes. Finally, cortisol pretreatment followed by P. salmonis challenge differentially modulated stress- and immune-related genes. These data suggest that fish muscle cells possess an intrinsic immune response and are differentially regulated by cortisol, which could lead to bacterial outbreaks in muscle under stress conditions.



中文翻译:

皮质醇对受鲑鱼攻击的虹鳟(Oncorhynchus mykiss)肌管免疫样反应的影响

鲑鱼是智利水产养殖中具有很高商业价值的物种,肌肉是该行业的最终产品。在集约化养殖过程中,鱼可能会受到压力的影响,从而增加对感染的易感性。最近,我们报道了肌肉是免疫反应的一个重要焦点。然而,研究表明压力的免疫抑制作用仅在淋巴器官中,很少有关于肌肉和免疫的研究。因此,我们确定皮质醇与挑战鱼类肌管的免疫样反应的影响Piscirickettsia鲑进行三次审判。首先,虹鳟鱼肌肉原代培养物被培养并用皮质醇 (100 ng/mL) 处理 3 和 4 小时。其次,肌管受到鲑鱼的挑战(MOI 50) 4、6 和 8 小时。第三,肌肉细胞培养物用皮质醇预处理,然后用鲑鱼攻击。通过qPCR评估糖皮质激素途径和先天免疫的mRNA水平。皮质醇增加klf15水平并下调先天免疫相关tlr5m基因和抗菌肽。鲑鱼挑战上调了几个免疫相关基因。最后,皮质醇预处理,然后是鲑鱼挑战差异调节的压力和免疫相关基因。这些数据表明,鱼类肌肉细胞具有内在的免疫反应,并受到皮质醇的差异调节,这可能导致压力条件下肌肉中的细菌爆发。

更新日期:2021-05-04
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