Introgression of novel components of partial resistance to P. nicotianae can be used to increase the range, level, and durability of resistance to the black shank disease of tobacco. Here we describe the development and evaluation of BC₉F₃ sub-sub-NILs of elite flue-cured tobacco cultivar ‘K326’ carrying a favorable allele(s) for resistance at the Phn15.1 quantitative trait locus derived from cigar cultivar ‘Beinhart 1000.’ We were able to identify a series of genotypes with significantly improved black shank resistance relative to K326 and, importantly, that possessed a mutant allele of NtCPS2, a closely linked gene involved in the biosynthesis of Z-abienol (an undesirable flavor and aroma compound for flue-cured tobacco). Some evidence of a potential influence of Phn15.1 on resistance to bacterial wilt caused by Ralstonia solanacearum was also observed. The Beinhart 1000 allele(s) at Phn15.1 acted in an additive manner to affect resistance to black shank. No significant reductions in cured leaf yields were found to be associated with Phn15.1-mediated black shank resistance. Although not statistically significant, trends of slightly reduced physical cured leaf quality were observed as the zygosity of the Beinhart 1000 Phn15.1 allele(s) increased. Increases in percent total alkaloids and reductions in percent reducing sugars were also found as the zygosity at Phn15.1 increased. These changes probably did not result in cured leaf chemistry being outside of the bounds of commercial acceptability, however. The derived genetic materials enhanced for partial genetic resistance to black shank should be of value for future flue-cured tobacco cultivar development efforts.

中文翻译：

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评估在 Phn15.1 位点携带 Beinhart-1000 等位基因的烟草品系和杂交种的农艺和抗病特性

到部分抗性的新颖组件的渐渗P.烟草可用于增加的范围内，电平，和电阻的耐久性烟草黑胫病。在这里，我们描述了在来自雪茄品种 'Beinhart的Phn15.1数量性状基因座上携带有利等位基因抗性的优良烤烟品种 'K326'的 BC ₉ F ₃ sub-sub-NIL的开发和评估1000.' 我们能够鉴定出一系列相对于 K326 具有显着改善的黑胫病抗性的基因型，重要的是，它们具有NtCPS2的突变等位基因，这是一个与Z生物合成密切相关的基因。-abienol（烤烟的一种不良风味和香气化合物）。的潜在影响的一些证据Phn15.1导通电阻引起的细菌性枯萎病青枯雷尔氏菌中也观察到。Phn15.1 的 Beinhart 1000 等位基因以加性方式影响对黑胫病的抗性。未发现腌制叶产量的显着降低与Phn15.1介导的黑胫抗性相关。尽管没有统计显着性，但观察到物理腌制叶质量略有下降的趋势，因为 Beinhart 1000 Phn15.1 的合子性等位基因增加。随着Phn15.1的接合度增加，还发现总生物碱百分比增加和还原糖百分比减少。然而，这些变化可能不会导致腌制的叶子化学成分超出商业可接受的范围。对黑胫病的部分遗传抗性增强的衍生遗传材料应该对未来的烤烟品种开发工作有价值。