miRNAs are attractive factors in cancer research studies due to their important roles for regulating of gene expression. Because of miRNA-21 expression surplus in many types of cancers, so accurate identification is important. Increasing efforts have caused different methods to improve the sensitivity and specificity of detection. Present study is an attempt to report a new electrochemical label-free PNA-based bioassay for detection of miRNA-21. In this study, gold electrode was modified by gold nanoparticles to improve a functional PNA-based biosensor. The EDS and field emission scanning electron microscope (FE-SEM) were used to detect fabrication of biosensor. The electrochemical behavior of sensor was evaluated after inserting of acpcPNA probes and miRNA-21 on the stucture of electrode and analyzed essential parameters such as various concentration of target miRNA, hybridization time, reproducibility, stability, and applicability. The results of study demonstrated that engineered biosensor was successfully fabricated. The findings showed the highest amount of current in 5 minutes hybridization time, with suitable reproducibility and stability. This innovative miRNA-based biosensor presents a sensitive and specific method in fast and may be lab-on chip assay in future.

中文翻译：

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吡咯烷基肽核酸 (acpcPNA-T9) 探针与 AuNPs 的化学结合对 miRNA-21 的无标记监测：一种用于生物医学分析和 POC 诊断的新型生物传感平台

miRNAs 在癌症研究中是有吸引力的因素，因为它们在调节基因表达方面具有重要作用。由于 miRNA-21 在多种癌症中表达过剩，因此准确鉴定很重要。越来越多的努力导致了不同的方法来提高检测的灵敏度和特异性。本研究试图报告一种新的基于电化学无标记 PNA 的生物测定法，用于检测 miRNA-21。在这项研究中，金电极被金纳米粒子修饰，以改进基于 PNA 的功能性生物传感器。EDS和场发射扫描电子显微镜（FE-SEM）用于检测生物传感器的制造。在电极结构上插入acpcPNA探针和miRNA-21后，对传感器的电化学行为进行了评估，并分析了各种靶miRNA浓度、杂交时间、重现性、稳定性和适用性等基本参数。研究结果表明，工程生物传感器已成功制造。研究结果表明，在 5 分钟的杂交时间内电流量最大，具有合适的重现性和稳定性。这种创新的基于 miRNA 的生物传感器提供了一种快速灵敏且特异的方法，未来可能会在芯片上进行实验室检测。研究结果表明，在 5 分钟的杂交时间内电流量最大，具有合适的重现性和稳定性。这种创新的基于 miRNA 的生物传感器提供了一种快速灵敏且特异的方法，未来可能会在芯片上进行实验室检测。研究结果表明，在 5 分钟的杂交时间内电流量最大，具有合适的重现性和稳定性。这种创新的基于 miRNA 的生物传感器提供了一种快速灵敏且特异的方法，未来可能会在芯片上进行实验室检测。