This study involved qualitative and quantitative analyses of rosmarinic acid (synonym: labiatenic acid), apigenin and buddleoside by high-performance thin-layer chromatography (HPTLC). The thin-layer plate was a polyamide film and dichloromethane, ethyl acetate, and formic acid (6:5:1, V/V) were used as the mobile phase, spraying 2% aluminum trichloride ethanol solution, heating until the spots were clear; the scanning wavelength was 330 nm. The R_F values of rosmarinic acid, apigenin and buddleoside were 0.20 ± 0.05, 0.36 ± 0.05 and 0.59 ± 0.05, respectively. The linear ranges of rosmarinic acid, apigenin and buddleoside were 0.0900‒0.4540 μg, 0.0328‒0.1968 μg and 0.02005‒0.1203 μg, respectively. The linear correlation coefficients were 0.9942, 0.9931 and 0.9904, respectively. The linear correlation coefficients were 0.9958, 0.9931 and 0.9928, respectively. The average recoveries of rosmarinic acid, apigenin and buddleoside were 101.29%, 99.75% and 100.8%, respectively, with RSD values of 1.81% (n = 6), 1.80% (n = 6) and 2.66% (n = 6), respectively. Thus, HPTLC proved to be a simple, accurate, and rapid qualitative and quantitative method. Therefore, the appropriate method should be selected according to the specific experimental conditions.

这项研究涉及通过高效薄层色谱法（HPTLC）对迷迭香酸（同义词：迷迭酸），芹菜素和虎皮甙的定性和定量分析。薄板为聚酰胺薄膜，以二氯甲烷，乙酸乙酯和甲酸（6：5：1，V / V）为流动相，喷雾2％三氯化铝乙醇溶液，加热直至斑点清晰; 扫描波长为330nm。在[R _˚F迷迭香酸，芹菜素和虎皮苷的值分别为0.20±0.05、0.36±0.05和0.59±0.05。迷迭香酸，芹菜素和虎皮苷的线性范围分别为0.0900‒0.4540μg，0.0328‒0.1968μg和0.02005‒0.1203μg。线性相关系数分别为0.9942、0.9931和0.9904。线性相关系数分别为0.9958、0.9931和0.9928。迷迭香酸，芹菜素和虎皮甙的平均回收率分别为101.29％，99.75％和100.8％，RSD值为1.81％（n = 6），1.80％（n = 6）和2.66％（n= 6）。因此，HPTLC被证明是一种简单，准确，快速的定性和定量方法。因此，应根据具体的实验条件选择合适的方法。