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Myristoylation alone is sufficient for PKA catalytic subunits to associate with the plasma membrane to regulate neuronal functions [Cell Biology]
Proceedings of the National Academy of Sciences of the United States of America ( IF 9.412 ) Pub Date : 2021-04-13 , DOI: 10.1073/pnas.2021658118
Wei-Hong Xiong, Maozhen Qin, Haining Zhong

Myristoylation is a posttranslational modification that plays diverse functional roles in many protein species. The myristate moiety is considered insufficient for protein–membrane associations unless additional membrane-affinity motifs, such as a stretch of positively charged residues, are present. Here, we report that the electrically neutral N-terminal fragment of the protein kinase A catalytic subunit (PKA-C), in which myristoylation is the only functional motif, is sufficient for membrane association. This myristoylation can associate a fraction of PKA-C molecules or fluorescent proteins (FPs) to the plasma membrane in neuronal dendrites. The net neutral charge of the PKA-C N terminus is evolutionally conserved, even though its membrane affinity can be readily tuned by changing charges near the myristoylation site. The observed membrane association, while moderate, is sufficient to concentrate PKA activity at the membrane by nearly 20-fold and is required for PKA regulation of AMPA receptors at neuronal synapses. Our results indicate that myristoylation may be sufficient to drive functionally significant membrane association in the absence of canonical assisting motifs. This provides a revised conceptual base for the understanding of how myristoylation regulates protein functions.



中文翻译:

单独的肉豆蔻酰化足以使PKA催化亚基与质膜结合以调节神经元功能[细胞生物学]

肉豆蔻酰基化是翻译后修饰,其在许多蛋白质种类中起多种功能作用。肉豆蔻酸酯部分被认为不足以实现蛋白质-膜的缔合,除非存在其他膜亲和性基序,例如一系列带正电荷的残基。在这里,我们报道蛋白质激酶A催化亚基(PKA-C)的电中性N末端片段,其中肉豆蔻酰化是唯一的功能性基序,足以进行膜缔合。这种肉豆蔻酰化作用可以使一部分PKA-C分子或荧光蛋白(FPs)与神经元树突中的质膜结合。尽管可以通过改变肉豆蔻酰化位点附近的电荷很容易地调节其膜亲和力,但PKA-C N末端的净中性电荷在进化上是保守的。观察到的膜结合虽然适度,但足以将PKA活性集中在膜上近20倍,并且是神经元突触中AMPA受体的PKA调节所必需的。我们的结果表明,在没有典型辅助基序的情况下,肉豆蔻酰化可能足以驱动功能上重要的膜缔合。这为理解肉豆蔻酰化如何调节蛋白质功能提供了修订的概念基础。

更新日期:2021-04-06
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