Objective

Histamine derived from mast cells and basophils plays important roles in inducing allergic symptoms. Although T cells also produce histamine, the involvement of the histamine produced from T cells has remained enigmatic. We sought to reveal the roles of T helper 2 (Th2) cell-derived histamine in nasal allergic disorders.

Methods

The histamine production from Th2 cells was measured by EIA. The mRNA expression of histidine decarboxylase (HDC) was measured by real-time PCR. To investigate the roles of Th2 cell-derived histamine in vivo, we analyzed an antigen-specific Th2 cell transfer mouse model.

Results

Th2 cells produced histamine by T cell receptor stimulation, and these properties were specific for Th2 cells, but not Th1 cells and naïve CD4 T cells. The histamine produced from Th2 cells was involved in the infiltrations of Th2 cells in response to antigen exposure.

Conclusion

These results suggest that Th2 cell-derived histamine play important roles in nasal allergic disorders.

中文翻译：

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Th2细胞来源的组胺参与小鼠鼻Th2浸润

客观的

源自肥大细胞和嗜碱性粒细胞的组胺在诱发过敏症状中起重要作用。尽管 T 细胞也产生组胺，但 T 细胞产生的组胺的参与仍然是个谜。我们试图揭示 T 辅助 2 (Th2) 细胞衍生的组胺在鼻过敏性疾病中的作用。

方法

通过EIA测量来自Th2细胞的组胺产量。通过实时PCR测量组氨酸脱羧酶(HDC)的mRNA表达。为了研究 Th2 细胞来源的组胺在体内的作用，我们分析了抗原特异性 Th2 细胞转移小鼠模型。

结果

Th2 细胞通过 T 细胞受体刺激产生组胺，这些特性对 Th2 细胞是特异性的，但不是 Th1 细胞和幼稚 CD4 T 细胞。Th2 细胞产生的组胺参与了 Th2 细胞对抗原暴露的浸润。

结论

这些结果表明 Th2 细胞来源的组胺在鼻过敏性疾病中起重要作用。