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Strategy for the construction of a core collection for Pinus yunnanensis Franch. to optimize timber based on combined phenotype and molecular marker data
Genetic Resources and Crop Evolution ( IF 2 ) Pub Date : 2021-04-03 , DOI: 10.1007/s10722-021-01182-9
Xiaoli Wang , Zilin Cao , Chengjie Gao , Kun Li

The core collection construction strategies are critical for the conservation and utilization of germplasm resources. The combined data of phenotypic characteristics and SRAP markers in 780 samples were employed to construct a core collection for Pinus yunnanensis Franch. Based on geographical distribution, a total of four P. yunnanensis germplasm subsets were configured by improving a least distance stepwise sampling method at four sampling proportions. The representativeness of germplasm subsets for original germplasms were evaluated by quantitative and qualitative trait independence tests. For the quantitative traits test, the germplasm subset at a 30% sampling proportion was the best of four germplasm subsets by the mean t-test, the variance F-test, the frequency distribution χ2-test, the Shannon diversity index, and five effective evaluation parameters. For the qualitative traits test, there were significant differences between the original germplasm and germplasm subset at a 10% sampling proportion in the mean t-test of genetic diversity indicators. The genetic diversity within the population was significantly different between the original germplasm and germplasm subsets at 20% and 10% sampling proportions, respectively, in the variance F-test. The results of quantitative and qualitative traits tests revealed that the 30% sampling proportion was appropriate for the construction of the core collection.



中文翻译:

云南松松核心种质构建策略。根据组合的表型和分子标记数据优化木材

核心馆藏建设策略对于种质资源的保护和利用至关重要。利用780个样品的表型特征和SRAP标记数据相结合,构建了云南松的核心种质。基于地理分布,总共四个P. ý unnanensis种质子集是通过在四个采样比例提高至少距离逐步取样方法构成。通过定量和定性性状独立性测试评估了原始种质的种质子集的代表性。对于数量性状测试,按t均值t计,以30%的抽样比例进行的种质亚组是四个种质亚组中最好的-test,方差˚F -test,频率分布χ 2 -test,香农多样性指数,和五个有效的评价参数。对于定性特质测试,在遗传多样性指标的平均t检验中,原始种质和种质子集之间有显着差异,抽样比例为10%。在方差F检验中,原始种质和种质子集之间的种群内遗传多样性分别以20%和10%的采样比例显着不同。定量和定性特征测试的结果表明,30%的采样比例适用于核心种质的构建。

更新日期:2021-04-04
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