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Transcriptomic and metabolomic reprogramming in cotton after Apolygus lucorum feeding implicated in enhancing recruitment of the parasitoid Peristenus spretus
Journal of Pest Science ( IF 4.8 ) Pub Date : 2021-04-04 , DOI: 10.1007/s10340-021-01369-0
Xinzheng Huang , Junfeng Kou , Weixia Jing , Xiaoqiang Han , Danfeng Liu , Somayyeh Ghasemzadeh , Peiyao Sun , Wangpeng Shi , Yongjun Zhang

Different types of insect feeding can activate distinct plant resistance mechanisms and trigger the generation of specific volatile compounds. Transcriptomic changes and the genomic basis underlying plant defense in response to chewing herbivores or phloem-feeding insects have been relatively well investigated, while better insight into molecular mechanisms underlying the plant defense response, in particular, volatile emissions triggered by sap-feeding insects such as the green plant bug Apolygus lucorum is needed. Here, we monitored transcriptomic and volatile metabolomic changes in cotton over time during A. lucorum infestation. RNA-seq analysis showed that 1614 transcripts were differentially expressed (log2|Ratio| ≥ 1; q ≤ 0.05) in cotton leaves infested by A. lucorum. All differentially expressed genes (DEGs) in jasmonic acid (JA; 48 genes) and salicylic acid (SA; 5 genes) pathways were upregulated, highlighting a central role of JA in A. lucorum-induced signaling without attenuating the SA pathway. Moreover, all DEGs (30 genes) involved in herbivore-induced volatile biosynthesis were upregulated. Consistently, A. lucorum-induced cotton volatile blends and synthetic methyl salicylate significantly attracted the parasitoid Peristenus spretus. The present data indicated that cotton plants after A. lucorum infestation undergo rapid, extensive transcriptome reprogramming mediated by complex signaling networks in which the JA and SA pathways act synergistically, leading to a specific volatile profile involved in an indirect plant defense.



中文翻译:

饲喂Apolygus lucorum后棉花中的转录组和代谢组学重编程与增加寄生性Peristenus spretus的募集有关

不同类型的昆虫摄食可以激活不同的植物抗性机制并触发特定挥发性化合物的产生。相对咀嚼草食动物或韧皮部摄食昆虫的转录组学变化和植物防御的基因组基础已经得到了比较充分的研究,同时对植物防御反应的分子机制有更深入的了解,特别是由食汁昆虫(如需要绿色植物臭虫Apolygus lucorum。在这里,我们监测了A. lucorum侵染期间棉花随时间变化的转录组和挥发性代谢组学变化。RNA-seq分析显示差异表达了1614个转录物(log 2 | Ratio |≥1;q ≤0.05)在被A. lucorum侵染的棉叶中。茉莉酸(JA; 48个基因)和水杨酸(SA; 5个基因)途径中的所有差异表达基因(DEG)均被上调,突显了JA在A.lucorum诱导的信号传导中的核心作用,而不会减弱SA途径。此外,与草食动物诱导的挥发性生物合成有关的所有DEG(30个基因)均被上调。一致地,A。lucorum诱导的棉花挥发性混合物和合成的水杨酸甲酯显着吸引了寄生性的Peristenus spretus。目前的数据表明,A。lucorum之后的棉花植株 侵染经历了由复杂的信号网络介导的快速,广泛的转录组重编程,其中JA和SA途径协同作用,导致涉及间接植物防御的特定挥发性特征。

更新日期:2021-04-04
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