Demographic data including characterizations of population sex and age composition are of fundamental importance for effective management, especially for numerically depressed Great Lakes populations of imperiled species such as lake sturgeon (Acipenser fulvescens). The goal of this paper was to extend a recently reported Acipenserid-derived PCR-based genotyping test to determine sex of lake sturgeon. We demonstrate that the recently reported AllWSex2 primers amplified the female sex-specific region in lake sturgeon of known sex, consistent with a ZZ/ZW mode of inheritance. Sanger sequencing of female lake sturgeon PCR products matched published sequences from female sterlet (Acipenser ruthenus), showing 100% query cover and 98% (63/64) identity. The ability to provide a rapid, cost-effective, and unambiguous determination of sex for lake sturgeon will allow managers to determine compositional estimates of sex ratios during any season, and for individuals at any age or size, which is of great utility for species characterized by delayed sexual maturity and lacking external sexual dimorphisms.

包括种群性别和年龄构成特征的人口统计数据对于有效管理至关重要，特别是对于数量减少的大湖区濒危物种种群，例如湖鲟 ( Acipenser fulvescens )。本文的目的是扩展最近报道的基于 Acipenserid 的 PCR 基因分型测试，以确定湖鲟的性别。我们证明最近报道的 AllWSex2 引物扩增了已知性别的湖鲟雌性性别特异性区域，与 ZZ/ZW 遗传模式一致。雌性湖鲟 PCR 产物的 Sanger 测序与来自雌性鲟 ( Acipenser ruthenus) 的已发表序列相匹配)，显示 100% 的查询覆盖和 98% (63/64) 的身份。为湖鲟提供快速、具有成本效益和明确的性别确定的能力将使管理人员能够确定任何季节以及任何年龄或大小的个体的性别比例的组成估计，这对于已表征的物种非常有用由于性成熟延迟和缺乏外部性二态性。