Zygosaccharomyces sp. is an industrially important yeast for the production traditional fermented foods in Japan. At present, however, there is no easy method for mating Zygosaccharomyces sp. strains in the laboratory; furthermore, little is known about the expression of mating-type-specific genes in this yeast. Here, mating was observed when Zygosaccharomyces sp. was subjected to nitrogen-starvation conditions. The expression of mating-type-specific genes, Zygo STE6 and Zygo MFα1, was induced under nitrogen-starvation conditions, as confirmed by lacZ reporter assay. This expression was mating-type-specific: Zygo STE6 was expressed specifically for mating-type a, whereas and Zygo MFα1 was expressed specifically for mating-type α. Yeast strains Zygosaccharomyces rouxii DL2 and DA2, derived from type strain Z. rouxii CBS732, did not show mating even under nitrogen-starvation conditions. Gene sequencing revealed that the Zygo STE12 in Z. rouxii CBS732 has a frameshift mutation. Under nitrogen starvation, mating was observed in both DL2 and DA2 transformed with the wild-type Zygo STE12. The expression of Zygo STE6 in Z. rouxii DL2 transformed with wild-type Zygo STE12 under nitrogen-starvation conditions was confirmed by lacZ reporter assay. Collectively, these results revealed that, under nitrogen-starvation conditions, Zygosaccharomyces sp. can mate and mating-type-specific genes are expressed. Furthermore, Zygo Ste12 is essential for both mating and the expression of mating-type-specific genes in Zygosaccharomyces sp.

中文翻译：

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Zygosaccharomyces sp. 中交配和交配型特异性基因的调控。酵母

接合酵母属 是日本生产传统发酵食品的重要工业酵母。然而，目前还没有简单的方法来交配Zygosaccharomyces sp。实验室中的菌株；此外，人们对这种酵母中交配型特异性基因的表达知之甚少。在这里，当Zygosaccharomyces sp.时观察到交配。经受氮饥饿条件。交配型特异性基因Zygo STE6和Zygo MFα1 的表达在氮饥饿条件下被诱导，如lacZ报告基因测定所证实的。这种表达是交配型特异性的：Zygo STE6是专门为交配型表达的a，而Zygo MFα1专为交配型 α 表达。酵母菌株鲁氏接合酵母DL2和DA2，从型菌株衍生Z.鲁氏CBS732，没有显示出氮饥饿条件下甚至相配合。基因测序揭示了的Zygo STE12在Z.氏酵母CBS732有移码突变。在氮饥饿条件下，在用野生型Zygo STE12转化的 DL2 和 DA2 中均观察到交配。的表达的Zygo STE6中Z.鲁氏DL2与野生型转化的Zygo STE12氮饥饿条件下被证实lacZ启动记者分析。总的来说，这些结果表明，在氮饥饿条件下，接合酵母属。可以表达交配和交配类型特异性基因。此外，Zygo Ste12 对于Zygosaccharomyces sp. 中的交配和交配类型特异性基因的表达都是必不可少的。