当前位置: X-MOL 学术Cell. Mol. Biol. Lett. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Relationship between miR-378c and YY1 expression in patients with gastric cancer and the clinicopathological features
Cellular & Molecular Biology Letters ( IF 8.3 ) Pub Date : 2021-04-01 , DOI: 10.1186/s11658-021-00256-x
Lei Zhang 1 , Lei Zou 1 , Peng Sun 1
Affiliation  

The purpose of this study was to explore the clinical value of miR-378c and its target gene YY1 in gastric cancer. The TCGA database was employed to analyse miR-378c expression in gastric cancer. qRT-PCR was applied to identify miR-378c and YY1 in tissues and serum of patients suffering from gastric cancer. The association of miR-378c with the clinical data of patients with gastric cancer was analysed. Receiver operating characteristics (ROC) curve analysis was used to determine the diagnostic value of miR-378c and YY1 in gastric cancer, and analyse the relationship between miR-378c and YY1 and patients’ survival. Pearson’s test was applied to determine the association between miR-378c and YY1 in tissue and serum of patients. Dual-Luciferase Reporter assay was employed to examine the targeting association between miR-378c and YY1. Finally, independent prognostic factors was determined in patients with gastric cancer using Cox regression analysis. In the TCGA database, miR-378c was weakly expressed in gastric cancer. Overall, patients with low expression had a lower survival rate. The expression of miR-378c decreased and the expression of YY1 increased in cancer tissues and serum of tumour patients. In patients with low expression of miR-378c the tumour size was ≥ 5 cm. Low differentiation, high TNM staging and lymph node invasion rate increased significantly, but the 5-year survival rate decreased in the patients. miR-378c and YY1 had better diagnostic value in gastric cancer. TargetScan, miRDB, starBase and miRTarBase predicted that YY1 was a potential gene of miR-378c, and the Dual-Luciferase Reporter assay revealed that there was a targeting relationship between the two, which was proved by correlation analysis. Multivariate Cox analysis revealed that differentiation, TNM staging and miR-378c were independent prognostic factors for patients. MiR-378c is weakly expressed in gastric cancer patients and may be considered as a promising diagnostic and prognostic indicator for gastric cancer.

中文翻译:

胃癌患者miR-378c、YY1表达与临床病理特征的关系

本研究旨在探讨miR-378c及其靶基因YY1在胃癌中的临床价值。TCGA数据库用于分析胃癌中miR-378c的表达。应用qRT-PCR鉴定胃癌患者组织和血清中的miR-378c和YY1。分析了 miR-378c 与胃癌患者临床数据的关联。采用受试者工作特征(ROC)曲线分析确定miR-378c和YY1在胃癌中的诊断价值,分析miR-378c和YY1与患者生存的关系。应用 Pearson 检验来确定患者组织和血清中 miR-378c 和 YY1 之间的关联。双荧光素酶报告基因检测用于检查 miR-378c 和 YY1 之间的靶向关联。最后,使用 Cox 回归分析确定胃癌患者的独立预后因素。在TCGA数据库中,miR-378c在胃癌中弱表达。总体而言,低表达患者的存活率较低。miR-378c在肿瘤患者的癌组织和血清中表达降低,YY1表达升高。在 miR-378c 低表达的患者中,肿瘤大小≥ 5 cm。低分化、高TNM分期和淋巴结浸润率显着增加,但患者的5年生存率下降。miR-378c和YY1在胃癌中具有较好的诊断价值。TargetScan、miRDB、starBase和miRTarBase预测YY1是miR-378c的潜在基因,Dual-Luciferase Reporter分析表明两者存在靶向关系,相关性分析证明了这一点。多变量 Cox 分析显示分化、TNM 分期和 miR-378c 是患者的独立预后因素。MiR-378c 在胃癌患者中的表达较弱,可能被认为是一种很有前景的胃癌诊断和预后指标。
更新日期:2021-04-02
down
wechat
bug