The house fly, Musca domestica L. is an important mechanical vector of different pathogens of medical and veterinary importance. It is an organism well-known for its ability to develop insecticide resistance. In the current study, we investigated the genetic basis and mechanism of chlorantraniliprole resistance in a field strain of house fly by selecting it artificially in the laboratory with a commercial formulation of chlorantraniliprole (CTPR-SEL). After seven generations of consecutive selection with chlorantraniliprole, CTPR-SEL strain developed a 644-fold resistance compared with the Susceptible strain and a 3-fold resistance compared with the field strain. Reciprocal crossing between the CTPR-SEL and Susceptible homozygous strains revealed an autosomal and incomplete dominant mode of resistance to chlorantraniliprole. A direct test using a monogenic inheritance model based on chi-square analysis revealed that the resistance was governed by more than one gene. Bioassays with synergists indicated that esterases might be involved in the resistance of house fly to chlorantraniliprole. These findings may be helpful to the development of an improved strategy for chlorantraniliprole resistance management in house fly.

家蝇，家蝇乳杆菌是具有医学和兽医学重要性的不同病原体的重要机械载体。它是一种有机体，具有产生抗药性的能力。在当前的研究中，我们通过在实验室中使用商业配方的扑虫腈（CTPR-SEL）人工选择它，研究了家蝇田间菌株对扑虫腈的抗性的遗传基础和机理。经过七代氯氰菊酯连续筛选后，CTPR-SEL菌株的抗药性比敏感菌株高644倍，抗药性比田间菌株高3倍。CTPR-SEL和易感纯合菌株之间的相互交叉揭示了对氯蒽醌的抗性的常染色体和不完全优势模式。使用基于卡方分析的单基因遗传模型进行的直接测试显示，抗性由一个以上的基因控制。协同增效剂的生物测定表明，酯酶可能参与了家蝇对绿藻腈的抗性。这些发现可能有助于制定改进的家蝇对氯氰菊酯抗性管理策略。