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Biocompatible N-doped carbon dots for the eradication of methicillin-resistant S. aureus (MRSA) and sensitive analysis for europium (III)
Nano-Structures & Nano-Objects Pub Date : 2021-04-01 , DOI: 10.1016/j.nanoso.2021.100724
Moorthy Maruthapandi , Poushali Das , Arumugam Saravanan , Michal Natan , Ehud Banin , Sriram Kannan , Shulamit Michaeli , John H.T. Luong , Aharon Gedanken

Plant leaves have more attracted in the preparation of carbon dots (CDs) owing to natural phytochemicals. Herein, we performed a one-pot hydrothermal process for the synthesis of Nitrogen doped CDs (N@CDs) from glossy heart-shaped Piper betel leaves (P. betel). The prepared N@CDs were effectively applied for the eradication of E. coli, S. aureus, and methicillin-resistant S. aureus (MRSA). The N@CDs were also used as a sensor for lanthanides detection with notable selectivity for Eu3+. The results revealed that the water-soluble N@CDs displayed narrow size particle distribution with a mean diameter of 2.6 nm. The bright fluorescence was estimated to be at λex: 350 with λem: 430 nm. The functional groups on N@CDs were confirmed by FTIR and XPS. The antimicrobial effect of N@CDs was concentration-dependent and effectively against E. coli and S. aureus at 1000 μg/mL. The antibacterial results reveal that the N@CDs exhibit the selective and superior killing effect against antibiotic-resistant bacteria (MRSA). The biocompatibility of CDs explored using THP- 1 monocytes cells. The N@CDs were also applicable for sensitive and selective detection of Eu3+ with LOD of 68 μM over other lanthanides in an aqueous solution by fluorescence quenching.



中文翻译:

生物相容性的氮掺杂碳点,用于消除耐甲氧西林的金黄色葡萄球菌(MRSA)和sensitive(III)的灵敏分析

由于天然植物化学成分,植物叶片在碳点(CD)的制备中更具吸引力。在这里,我们执行了一锅水热法,从有光泽的心形Piper槟榔叶(P. betel)合成了氮掺杂的CD(N @ CDs )。制备的N @ CDs有效地用于根除大肠杆菌金黄色葡萄球菌和耐甲氧西林的金黄色葡萄球菌(MRSA)。N @ CD还用作镧系元素检测的传感器,对Eu 3+具有显着的选择性。结果表明,水溶性N @ CDs的粒径分布较窄,平均直径为2.6 nm。明亮的荧光估计为λ前任:350与 λEM:430nm。N @ CDs上的官能团已通过FTIR和XPS确认。N @ CDs的抗菌作用是浓度依赖性的,并在1000 ℃时有效抵抗大肠杆菌金黄色葡萄球菌μ克/毫升。抗菌结果表明,N @ CD对抗生素抗性细菌(MRSA)表现出选择性和优异的杀灭作用。使用THP-1单核细胞探索CD的生物相容性。N @ CD也适用于灵敏度和选择性检测Eu 3 +,LOD为68μ通过荧光猝灭使水溶液中的M超过其他镧系元素。

更新日期:2021-04-02
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