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Effects of antifungal agents on the fungal proteome: informing on mechanisms of sensitivity and resistance
Expert Review of Proteomics ( IF 3.4 ) Pub Date : 2021-04-12 , DOI: 10.1080/14789450.2021.1912601
Rebecca A Owens 1, 2 , Sean Doyle 1
Affiliation  

ABSTRACT

Introduction

Antifungal agents are essential in the fight against serious fungal disease, however emerging resistance is threatening an already limited collection of therapeutics. Proteomic analyses of effects of antifungal agents can expand our understanding of multifactorial mechanisms of action and have also proven valuable to elucidate proteomic changes associated with antifungal resistance.

Areas covered

This review covers the application of proteomic techniques to examine sensitivity and resistance to antifungals including commonly used therapeutics, amphotericin B, echinocandins and the azoles, based predominantly on studies involving Aspergillus fumigatus, Candida albicans and Candida glabrata from the last 10 years. In addition, non-clinical antimicrobial agents are also discussed, which highlight the potential of proteomics to identify new antifungal targets.

Expert commentary

Fungal proteomics has evolved in the last decade with increased genome availability and developments in mass spectrometry. Collectively, these have led to the advancement of proteomic techniques, allowing increased coverage of the proteome. Gel-based proteomics laid the foundation for these types of studies, which has now shifted to the more powerful gel-free proteomics. This has resulted in the identification of key mediators and potential biomarkers of antifungal resistance, as well as elucidating the mechanisms of action of novel and established antifungal agents.



中文翻译:

抗真菌剂对真菌蛋白质组的影响:关于敏感性和抗性机制的信息

摘要

介绍

抗真菌剂对于对抗严重的真菌病至关重要,但新出现的耐药性正在威胁已经有限的治疗方法。抗真菌药物作用的蛋白质组学分析可以扩大我们对多因素作用机制的理解,并且也证明对阐明与抗真菌药物耐药相关的蛋白质组学变化很有价值。

涵盖的领域

这篇综述主要基于过去 10 年涉及烟曲霉、白色念珠菌和光滑念珠菌的研究,涵盖了应用蛋白质组学技术检查抗真菌药物的敏感性和耐药性,包括常用的治疗药物、两性霉素 B、棘白菌素和唑类。此外,还讨论了非临床抗菌剂,突出了蛋白质组学识别新抗真菌靶点的潜力。

专家评论

真菌蛋白质组学在过去十年中随着基因组可用性的增加和质谱技术的发展而发展。总的来说,这些都导致了蛋白质组技术的进步,从而增加了蛋白质组的覆盖范围。基于凝胶的蛋白质组学为这些类型的研究奠定了基础,现在已经转向更强大的无凝胶蛋白质组学。这导致了抗真菌抗性的关键介质和潜在生物标志物的鉴定,以及阐明新的和成熟的抗真菌药物的作用机制。

更新日期:2021-05-14
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