Denitrification is a potential strategy for nitrate removal from wastewater. This study reports the isolation of a novel bacterium Georgenia daeguensis ARB2 which has not been earlier reported for treatment of wastewater. ARB2 was isolated from pharmaceutical wastewater and optimized for growth and nitrate removal under conditions of 10 mM nitrate stress (140 mgL⁻¹ \({{\text{NO}}_{3}^{ - }} - {\text{N}}\)). It can utilize nitrate as a sole source of nitrogen, exhibits highest growth and aerobic denitrification using glycogen + maltose as a carbon source at pH 7.0, carbon-to-nitrogen ratio of 34, temperature 30 °C, and shaking speed of 100 rpm. ARB2 removes 20% of the initial nitrate concentration of 10 mM in 56 h, and 40% of the initial nitrate concentration of 1 mM in 52 h. Further, the efficacy of strain ARB2 was tested on real contaminated waters and was found to successfully reduce nitrate levels in them. The findings suggest that Georgenia daeguensis ARB2 has a potential application for the alleviation of nitrate under aerobic conditions.

反硝化是从废水中去除硝酸盐的潜在策略。这项研究报告了一种新型细菌Georgia daeguensis ARB2的分离，该细菌尚未较早报道用于废水处理。从制药废水中分离出ARB2，并针对10 mM硝酸盐胁迫（140 mgL ^-1 \（{{\ text {NO}} _ {3} ^ {-}}-{\ text {N }} \））。它可以利用硝酸盐作为唯一的氮源，使用糖原+麦芽糖作为碳源，在pH 7.0，碳氮比为34，温度为30°C和振摇速度为100 rpm时，表现出最高的生长和好氧反硝化作用。ARB2在56小时内去除了10 mM的初始硝酸盐浓度的20％，在52小时内去除了1 mM的初始硝酸盐浓度的40％。此外，在真实的污水中测试了ARB2菌株的功效，发现该菌株成功降低了其中的硝酸盐含量。这些发现表明，Georgia daeguensis ARB2在缓解好氧条件下的硝酸盐方面具有潜在的应用价值。