Microbial communities play fundamental roles in association with marine algae; in fact, they are recognized to be actively involved in growth and morphogenesis of the algae. Porphyra purpurea is a red alga commonly found in the intertidal zone with a high economic value, however little is known about the bacterial species associated with this genus. Here we report the bacterial-associated diversity of P. purpurea in four different localities (Ireland, Italy United Kingdom and the USA) from analyzing eight publicly available metagenomic datasets. These were analyzed with Methaplan3 to identify the putative bacterial taxonomies and their relative abundances. Furthermore, we compared these results to the 16S rRNA metagenomic analysis pipeline of the MGnify database to evaluate both methods. Kraken2 was used to verify and support the results, as a complementary classification method to Metaphlan3. This approach highlighted the different taxonomic resolution of a 16S rRNA OTU-based method compared to the pan-genome approach deployed by Metaphlan3 and complemented by Kraken2. The results presented here provide valuable preliminary data on the putative host-associated bacterial species of P. purpurea.

微生物群落在与海藻相关的过程中发挥着重要作用；事实上，它们被认为积极参与藻类的生长和形态发生。Porphyra purpurea是一种常见于潮间带的红藻，具有很高的经济价值，但对与该属相关的细菌种类知之甚少。在这里，我们报告了P. purpurea的细菌相关多样性在四个不同的地方（爱尔兰、意大利、英国和美国）通过分析八个公开可用的宏基因组数据集。用 Methaplan3 对这些进行分析，以确定假定的细菌分类法及其相对丰度。此外，我们将这些结果与 MGnify 数据库的 16S rRNA 宏基因组分析管道进行了比较，以评估这两种方法。Kraken2 用于验证和支持结果，作为 Metaphlan3 的补充分类方法。与 Metaphlan3 部署并由 Kraken2 补充的泛基因组方法相比，这种方法突出了基于 16S rRNA OTU 的方法的不同分类分辨率。此处介绍的结果提供了关于P. purpurea的假定宿主相关细菌种类的有价值的初步数据。