The importance of studying storage alterations in erythrocytes is highlighted by the need to understand changes that could potentially serve to optimize the storage system. With this aim, the non-equilibrium thermodynamic theory with internal variables was introduced, and perturbing the erythrocyte samples with a harmonic electric field some functions of the theory have been determined varying in the perturbation frequency. A frequency has been noted that acts as a separator element between two states showing a different entropy production above and below this frequency. In stored red blood cells compared to fresh ones, the increase in entropy production measured shows a greater state of disorder in the latter. Further alterations have been highlighted on the surface charge density of the stored erythrocyte membrane and on the speed of anionic kinetics. All these observations highlight the image of membrane structural and functional alterations of the stored erythrocytes and lead to the elaboration of a technique able to correlate a specific perturbation frequency with the aging time of red blood cells.

中文翻译：

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红细胞悬浮液和Band 3蛋白的氧脱氧功能的热力学表征：对比研究

需要了解可能潜在地用于优化存储系统的更改，凸显了研究红细胞存储更改的重要性。为此目的，引入了具有内部变量的非平衡热力学理论，并利用谐波电场对红细胞样本进行了扰动，确定了该理论的某些功能，其扰动频率有所不同。已经注意到一个频率，该频率充当两个状态之间的分隔元素，在该频率之上和之下显示出不同的熵产生。与储存的红细胞相比，在储存的红细胞中，所测得的熵产生增加表明后者的状态更加混乱。在所存储的红细胞膜的表面电荷密度和阴离子动力学的速度上，其他变化已被强调。所有这些观察结果都突出显示了所储存的红细胞的膜结构和功能改变的图像，并导致了一种能够将特定的摄动频率与红细胞的衰老时间相关联的技术的完善。