Topical formulations containing 1–2% of Pinus brutia bark extract and Pycnogenol® have been prepared to investigate the effect of flavonoids on the stability of O/W emulsions, which were subjected to physicochemical and thermal stability tests. The formulations have been applied to cotton fabrics to evaluate antimicrobial properties against Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus brasiliensis. Furthermore, prepared cotton fabrics have been tested on keratinocytes seeded in cell culture inserts for wound healing. Results of freeze thaw cycle test indicated enhanced thermo-stability with no major changes in pH and viscosity, likewise the results of centrifugation assay. However, the addition of Pycnogenol® has tremendously decreased the viscosity of the topical formulation (10,900 cp.). In terms of antimicrobial activity, 2% P. brutia treated cotton fabrics decreased the proliferation of Aspergillus brasiliensis 78.8%, which were more effective than that of Pycnogenol® formulation (62.9%). As for wound healing, 2% P. brutia treated cotton fabrics increased HaCaT keratinocyte cell proliferation and accelerated the cell-free gap closure compared to Pycnogenol® and untreated control groups. The obtained results indicate the utilization of pine bark for developing an eco-friendly natural antifungal finish for medical textiles.

已制备含有 1-2% 的红松树皮提取物和碧容健® 的外用制剂，以研究黄酮类化合物对 O/W 乳液稳定性的影响，并对其进行了物理化学和热稳定性测试。该配方已应用于棉织物，以评估对金黄色葡萄球菌、大肠杆菌、白色念珠菌和巴西曲霉的抗菌性能. 此外，已制备的棉织物已在接种于细胞培养插入物中的角质形成细胞上进行了测试，用于伤口愈合。冻融循环测试的结果表明，热稳定性增强，pH 和粘度没有重大变化，离心测定的结果也是如此。然而，碧容健® 的加入极大地降低了外用制剂的粘度 (10,900 cp.)。在抗菌活性方面，2% P. brutia处理的棉织物减少了 78.8% 的巴西曲霉增殖，比碧容健® 配方 (62.9%) 更有效。至于伤口愈合，2% P. brutia与碧容健®和未处理的对照组相比，处理过的棉织物增加了 HaCaT 角质形成细胞的增殖并加速了无细胞间隙的闭合。所得结果表明松树皮可用于开发用于医用纺织品的环保天然抗真菌整理剂。