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GENETIC AND MORPHOLOGIC CHARACTERIZATION OF DIASCHISTORCHIS PANDUS (DIGENEA: PRONOCEPHALIDAE) TREMATODES EXTRACTED FROM HAWKSBILL TURTLES, ERETMOCHELYS IMBRICATA (TESTUDINES: CHELONIIDAE), IN GRENADA, WEST INDIES
Journal of Parasitology ( IF 1.3 ) Pub Date : 2021-03-29 , DOI: 10.1645/20-54
Daniel M. Fitzpatrick 1 , Monica A. Tetnowski 1 , Thomas G. Rosser 2 , Rhonda D. Pinckney 1 , David P. Marancik 1 , Brian P. Butler 1
Affiliation  

The hawksbill turtle Eretmochelys imbricata is a critically endangered species with a worldwide distribution. Limited information is available about the naturally occurring intestinal parasites of this species and what impact these parasites may have on the health of the hawksbill turtle. Diaschistorchis pandus was identified postmortem in 5 hawksbill turtles from Grenada, West Indies, using morphologic characterization. Sanger sequencing was performed for conserved ribosomal regions (5.8S, ITS2, 28S) and the mitochondrial cytochrome c oxidase subunit 1 gene (COI). Phylogenetic analysis of the 28S rRNA gene sequence data shows D. pandus clustering with other trematodes in the family Pronocephalidae, corroborating morphological classification. No genetic sequences have been previously reported for this trematode species, which has limited the collection of objective epidemiological data about this parasite of marine turtles.



中文翻译:

西印度群岛格林纳达的鹰嘴豆HA(Hawksbill turtles),RET蝶(Istememochelys Imbricata)(TESCHEDINESSE:CHELONIIDAE)中提取的腹泻(Dischistorchis Pandus,Digeneea:Pronocephalidae)气管的遗传和形态学特征。

玳瑁Eretmochelys玳瑁是一种极度濒危的物种分布在全世界。关于该物种天然存在的肠道寄生虫以及这些寄生虫可能对turtle的健康产生何种影响的信息有限。使用形态学特征,在西印度群岛格林纳达的5头post中发现了Pandias鼠疫。用于保守核糖体区域进行Sanger测序(5.8SITS228S)和线粒体细胞色素C ^氧化酶亚基1个基因(COI)。系统发育分析的28S rRNA基因序列数据显示D. pandusPronocephalidae科中的其他吸虫聚在一起,证实了形态学上的分类。以前没有关于这种吸虫物种的遗传序列的报道,这限制了有关这种海龟寄生虫的客观流行病学数据的收集。

更新日期:2021-03-31
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