ABSTRACT

Characterization of the morphometric traits of crustacean larvae is the basis for comparative works in many fields, including ecology, fisheries and aquaculture. Often, field sampled specimens will be stored in preservative before being sorted in the laboratory. Length changes are significant in fish larvae stored in different preservatives. However, no comparative study has been reported in crustacean larvae. This work investigates the effect of different measuring techniques, preservation methods and storage duration on the morphometric measurements of mud crab Scylla olivacea and the giant freshwater prawn Macrobrachium rosenbergii larvae. Measurements did not differ significantly when measured with stereo microscope or advanced research microscope. Different storage methods (70% ethanol, 95% ethanol and 10% neutral buffered formalin) did not result in inconsistency in the total length (TL) and dorsal spine (DS) of S. olivacea larvae, and the rostral spine (RS) of M. rosenbergii larvae. TL of M. rosenbergii larvae was significantly shorter when stored in 95% ethanol. Significant shrinkage of crustacean larvae was observed during the first few days after immersion in preservatives.

摘要

甲壳类幼虫形态特征的表征是在生态，渔业和水产养殖等许多领域进行比较研究的基础。通常，野外采样的样本会在实验室进行分类之前先保存在防腐剂中。储存在不同防腐剂中的鱼幼虫的体长变化很明显。但是，没有关于甲壳类幼虫的比较研究的报道。这项工作研究了不同的测量技术，保存方法和保存时间对泥蟹小锦葵和巨型淡水虾罗氏沼虾的形态测量的影响。幼虫。使用体视显微镜或高级研究显微镜进行测量时，测量结果无显着差异。不同的贮藏方法（70％乙醇，95％乙醇和10％中性福尔马林缓冲液）不会导致S. olivacea幼虫的全长（TL）和背脊（DS）不一致，以及罗氏沼虾幼虫。当储存在95％的乙醇中时，罗氏沼虾的幼虫的TL明显较短。浸入防腐剂后的头几天，甲壳类幼虫明显萎缩。